마우스(Mouse) IL11 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

IL11 cDNA Clone Datasheet

IL11 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008350.2
• cDNA Size: 600
• Gene Synonym: IL-11
• Source: Mouse

IL11 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus interleukin 11 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

IL11 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL11 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

IL11 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACTGTG TTTGTCGCCT GGTCCTGGTG GTGCTGAGCC TCTGGCCAGA TAGAGTCGTT
61       GCCCCTGGGC CACCAGCTGG CTCCCCTCGA GTCTCTTCAG ACCCTCGAGC AGATCTGGAC
121      AGCGCTGTTC TCCTAACCCG ATCCCTCCTG GCAGACACAC GGCAACTAGC TGCACAGATG
181      AGAGACAAAT TCCCAGCTGA CGGAGATCAC AGTCTGGACT CCCTGCCCAC CTTGGCCATG
241      AGCGCTGGGA CATTGGGATC TTTGCAGCTT CCTGGTGTGC TGACAAGGCT TCGAGTAGAC
301      TTGATGTCCT ACCTCCGGCA TGTACAATGG CTGCGCCGTG CAGGTGGTCC TTCCCTAAAG
361      ACTCTGGAGC CAGAGCTGGG TGCCCTGCAA GCCCGACTGG AACGGCTACT CCGCCGTTTA
421      CAGCTCTTGA TGTCTCGCCT GGCCTTGCCC CAGGCAGCCC CAGACCAACC TGTGATCCCC
481      CTGGGCCCTC CTGCCTCAGC CTGGGGAAGC ATCCGGGCAG CTCATGCCAT CCTAGGAGGG
541      CTGCACCTGA CCTTGGACTG GGCCGTGCGG GGCCTGCTGT TGTTAAAGAC TCGACTGTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).