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휴먼(Human) IL18BPa transcript variant E 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)PDF Download

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HG10357-M
  YES       
Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_001039659.1

cDNA Size:   579

Synonym:  IL18BPa

Source:  Human. cDNA Description:  ORF Clone of Homo sapiens interleukin 18 binding protein (IL18BP), transcript variant E DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the 575G/T and 577T/A mutations which cause the 192Gly and stop codon to be substituted by Val and Lys respectively. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
  1. Remove the marked edge in the center of the spot .
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to a heating block at 95oC for 15–30 min .
  4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGAGACACA ACTGGACACC AGACCTCAGC CCTTTGTGGG TCCTGCTCCT GTGTGCCCAC
61 GTCGTCACTC TCCTGGTCAG AGCCACACCT GTCTCGCAGA CCACCACAGC TGCCACTGCC
121 TCAGTTAGAA GCACAAAGGA CCCCTGCCCC TCCCAGCCCC CAGTGTTCCC AGCAGCTAAG
181 CAGTGTCCAG CATTGGAAGT GACCTGGCCA GAGGTGGAAG TGCCACTGAA TGGAACGCTG
241 AGCTTATCCT GTGTGGCCTG CAGCCGCTTC CCCAACTTCA GCATCCTCTA CTGGCTGGGC
301 AATGGTTCCT TCATTGAGCA CCTCCCAGGC CGACTGTGGG AGGGGAGCAC CAGCCGGGAA
361 CGTGGGAGCA CAGGTACGCA GCTGTGCAAG GCCTTGGTGC TGGAGCAGCT GACCCCTGCC
421 CTGCACAGCA CCAACTTCTC CTGTGTGCTC GTGGACCCTG AACAGGTTGT CCAGCGTCAC
481 GTCGTCCTGG CCCAGCTCTG GGCTGGGCTG AGGGCAACCT TGCCCCCCAC CCAAGAAGCC
541 CTGCCCTCCA GCCACAGCAG TCCACAGCAG CAGGTTAAA

Quality Control:   cDNA sequencing results is available upon request.


Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).