IL-33 Protein, Human, Recombinant

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IL-33 Protein, Human, Recombinant: Product Information

Purity
> 98 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 μg/ml (100 μl/well) can bind human IL1RL1 with a linear range of 0.31-5 ng/ml.
Protein Construction
A DNA sequence encoding the C-terminal segment of human IL33 (NP_254274.1) (Ser 112-Thr 270) was expressed with an initial Met at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human IL33 comprises 160 amino acids and predicts a molecular mass of 18 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-33 Protein, Human, Recombinant: Images

IL-33 Protein, Human, Recombinant: Alternative Names

C9orf26 Protein, Human; DVS27 Protein, Human; IL1F11 Protein, Human; NF-HEV Protein, Human; NFEHEV Protein, Human

IL-33 Background Information

Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial enules), is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.
Full Name
interleukin 33
References
  • Iikura M, et al. (2007) IL-33 can promote survival, adhesion and cytokine production in human mast cells. Lab Invest. 87(10): 971-8.
  • Lamkanfi M, et al. (2009) IL-33 raises alarm. Immunity. 31(1): 5-7.
  • Altered serum levels of IL-33 in patients with advanced systolic chronic heart failure: correlation with oxidative stress
    Author
    Zhang, HF;Xie, SL;Chen, YX;Mai, JT;Wang, JF;Zhu, WL;Zhu, LG;
    Year
    2012
    Journal
    J Transl Med
    Application
    cell-based (culture)
  • IL-33 signaling fuels outgrowth and metastasis of human lung cancer
    Author
    Wang, C;Chen, Z;Bu, X;Han, Y;Shan, S;Ren, T;Song, W;
    Year
    2016
    Journal
    Biochem. Biophys. Res. Commun.
    Application
    cell-based
  • Human S100A7 Induces Mature Interleukin1α Expression by RAGE-p38 MAPK-Calpain1 Pathway in Psoriasis
    Author
    Lei, H;Li, X;Jing, B;Xu, H;Wu, Y;
    Year
    2017
    Journal
    PLoS ONE
    Application
    cell-based
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