마우스(Mouse) IL33/NF-HEV 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

IL33 cDNA Clone Datasheet

IL33 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_133775.1
• cDNA Size: 801
• Gene Synonym: Il-33; Il1f11; NF-HEV; 9230117N10Rik
• Source: Mouse

IL33 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus interleukin 33 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

IL33 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL33 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

IL33 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGACCTA GAATGAAGTA TTCCAACTCC AAGATTTCCC CGGCAAAGTT CAGCAGCACC
61       GCAGGCGAAG CCCTGGTCCC GCCTTGCAAA ATAAGAAGAT CCCAACAGAA GACCAAAGAA
121      TTCTGCCATG TCTACTGCAT GAGACTCCGT TCTGGCCTCA CCATAAGAAA GGAGACTAGT
181      TATTTTAGGA AAGAACCCAC GAAAAGATAT TCACTAAAAT CGGGTACCAA GCATGAAGAG
241      AACTTCTCTG CCTATCCACG GGATTCTAGG AAGAGATCCT TGCTTGGCAG TATCCAAGCA
301      TTTGCTGCGT CTGTTGACAC ATTGAGCATC CAAGGAACTT CACTTTTAAC ACAGTCTCCT
361      GCCTCCCTGA GTACATACAA TGACCAATCT GTTAGTTTTG TTTTGGAGAA TGGATGTTAT
421      GTGATCAATG TTGACGACTC TGGAAAAGAC CAAGAGCAAG ACCAGGTGCT ACTACGCTAC
481      TATGAGTCTC CCTGTCCTGC AAGTCAATCA GGCGACGGTG TGGATGGGAA GAAGCTGATG
541      GTGAACATGA GTCCCATCAA AGACACAGAC ATCTGGCTGC ATGCCAACGA CAAGGACTAC
601      TCCGTGGAGC TTCAAAGGGG TGACGTCTCG CCTCCGGAAC AGGCCTTCTT CGTCCTTCAC
661      AAAAAGTCCT CGGACTTTGT TTCATTTGAA TGCAAGAATC TTCCTGGCAC TTACATAGGA
721      GTAAAAGATA ACCAGCTGGC TCTAGTGGAG GAGAAAGATG AGAGCTGCAA CAATATTATG
781      TTTAAGCTCT CGAAAATCTA A

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).