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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) IL33/NF-HEV 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) IL33/NF-HEV 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10368-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_033439.2
cDNA Size: 813
Synonym: DVS27, NF-HEV, NFEHEV, C9orf26, DKFZp586H0523, RP11-575C20.2
Source: Human. cDNA Description: ORF Clone of Homo sapiens interleukin 33 DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the 489C/T mutation not causing amino acid variation. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
Gene Bank Ref.ID: NM_033439.2
cDNA Size: 813
Synonym: DVS27, NF-HEV, NFEHEV, C9orf26, DKFZp586H0523, RP11-575C20.2
Source: Human. cDNA Description: ORF Clone of Homo sapiens interleukin 33 DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the 489C/T mutation not causing amino acid variation. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGAAGCCTA AAATGAAGTA TTCAACCAAC AAAATTTCCA CAGCAAAGTG GAAGAACACA
61 GCAAGCAAAG CCTTGTGTTT CAAGCTGGGA AAATCCCAAC AGAAGGCCAA AGAAGTTTGC
121 CCCATGTACT TTATGAAGCT CCGCTCTGGC CTTATGATAA AAAAGGAGGC CTGTTACTTT
181 AGGAGAGAAA CCACCAAAAG GCCTTCACTG AAAACAGGTA GAAAGCACAA AAGACATCTG
241 GTACTCGCTG CCTGTCAACA GCAGTCTACT GTGGAGTGCT TTGCCTTTGG TATATCAGGG
301 GTCCAGAAAT ATACTAGAGC ACTTCATGAT TCAAGTATCA CAGGAATTTC ACCTATTACA
361 GAGTATCTTG CTTCTCTAAG CACATACAAT GATCAATCCA TTACTTTTGC TTTGGAGGAT
421 GAAAGTTATG AGATATATGT TGAAGACTTG AAAAAAGATG AAAAGAAAGA TAAGGTGTTA
481 CTGAGTTATT ATGAGTCTCA ACACCCCTCA AATGAATCAG GTGACGGTGT TGATGGTAAG
541 ATGTTAATGG TAACCCTGAG TCCTACAAAA GACTTCTGGT TGCATGCCAA CAACAAGGAA
601 CACTCTGTGG AGCTCCATAA GTGTGAAAAA CCACTGCCAG ACCAGGCCTT CTTTGTCCTT
661 CATAATATGC ACTCCAACTG TGTTTCATTT GAATGCAAGA CTGATCCTGG AGTGTTTATA
721 GGTGTAAAGG ATAATCATCT TGCTCTGATT AAAGTAGACT CTTCTGAGAA TTTGTGTACT
781 GAAAATATCT TGTTTAAGCT CTCTGAAACT TAG
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
