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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) IL6 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) IL6 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10395-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_000600.2
cDNA Size: 639
Synonym: HGF, HSF, BSF2, IL-6, IFNB2, IL6
Source: Human. cDNA Description: ORF Clone of Homo sapiens interleukin 6 (interferon, beta 2) DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 429C/G not causing amino acid variation. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
Gene Bank Ref.ID: NM_000600.2
cDNA Size: 639
Synonym: HGF, HSF, BSF2, IL-6, IFNB2, IL6
Source: Human. cDNA Description: ORF Clone of Homo sapiens interleukin 6 (interferon, beta 2) DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 429C/G not causing amino acid variation. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGAACTCCT TCTCCACAAG CGCCTTCGGT CCAGTTGCCT TCTCCCTGGG GCTGCTCCTG
61 GTGTTGCCTG CTGCCTTCCC TGCCCCAGTA CCCCCAGGAG AAGATTCCAA AGATGTAGCC
121 GCCCCACACA GACAGCCACT CACCTCTTCA GAACGAATTG ACAAACAAAT TCGGTACATC
181 CTCGACGGCA TCTCAGCCCT GAGAAAGGAG ACATGTAACA AGAGTAACAT GTGTGAAAGC
241 AGCAAAGAGG CACTGGCAGA AAACAACCTG AACCTTCCAA AGATGGCTGA AAAAGATGGA
301 TGCTTCCAAT CTGGATTCAA TGAGGAGACT TGCCTGGTGA AAATCATCAC TGGTCTTTTG
361 GAGTTTGAGG TATACCTAGA GTACCTCCAG AACAGATTTG AGAGTAGTGA GGAACAAGCC
421 AGAGCTGTGC AGATGAGTAC AAAAGTCCTG ATCCAGTTCC TGCAGAAAAA GGCAAAGAAT
481 CTAGATGCAA TAACCACCCC TGACCCAACC ACAAATGCCA GCCTGCTGAC GAAGCTGCAG
541 GCACAGAACC AGTGGCTGCA GGACATGACA ACTCATCTCA TTCTGCGCAG CTTTAAGGAG
601 TTCCTGCAGT CCAGCCTGAG GGCTCTTCGG CAAATGTAG
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
