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휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag 태그

데이터시트리뷰관련제품프로토콜
Human IL11RA cDNA 클론 제품 정보
Gene_bank_ref_id:NM_004512.3
cDNA 크기:1269bp
cDNA 설명:Full length Clone DNA of Homo sapiens interleukin 11 receptor, alpha (IL11RA), transcript variant 1 with Flag tag.
유전자 동의어:IL11RA, MGC2146
:Human
벡터:pCMV2-FLAG
Plasmid:pCMV2-IL11RA-flag
제한 사이트:KpnI + XhoI (5.4kb + 1.32kb)
태그 씨퀀스:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
염기서열 설명:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
pCMV2-FLAG Vector Information
 
Vector Name pCMV2-FLAG
Vector Size 5592bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag FLAG
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

Schematic of pCMV2-FLAG Multiple Cloning Sites

FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag 태그 on other vectors
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그HG10252-ACG265260
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그HG10252-ACR265260
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag 태그HG10252-CF229890
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His 태그HG10252-CH229890
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc 태그HG10252-CM229890
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA 태그HG10252-CY229890
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone in cloning vectorHG10252-M88420
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag 태그HG10252-M-F229890
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag 태그HG10252-NF229890
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His 태그HG10252-NH229890
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc 태그HG10252-NM229890
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA 태그HG10252-NY229890
휴먼 IL11RA/IL-11RA transcript variant 1 Gene ORF cDNA clone expression plasmidHG10252-UT229890
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Product nameProduct name
연구배경

Interleukin 11 receptor, alpha subunit (IL11RA/IL-11RA) is a subunit of the interleukin 11 receptor which is a member of the hematopoietic cytokine receptor family. IL11RA/IL-11RA is expressed in a number of cell lines, including the myelogenous leukemia cell line K562, the megakaryocytic leukemia cell line Mo7E, the erythroleukemia cell line TF1, and the osteosarcoma cell lines, MG-63 and Saos-2. It is also expressed in normal and malignant prostate epithelial cell lines. Expression levels are increased in prostate carcinoma. This particular receptor is very similar to ciliary neurotrophic factor, since both contain an extracellular region with a 2-domain structure composed of an immunoglobulin-like domain and a cytokine receptor-like domain. Alternative splicing has been observed at this locus, and three variants encoding two different isoforms have been identified. IL11RA/IL-11RA is a receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. Defects in IL11RA/IL-11RA are a cause of craniosynostosis and dental anomalies (CRSDA). CRSDA is a disorder characterized by craniosynostosis, maxillary hypoplasia, and dental anomalies, including malocclusion, delayed and ectopic tooth eruption, and/or supernumerary teeth. Some patients also display minor digit anomalies, such as syndactyly and/or clinodactyly.

참고자료
  • Van Leuven F, et al. (1996) Molecular cloning and characterization of the human interleukin-11 receptor alpha-chain gene, IL11RA, located on chromosome 9p13. Genomics. 31 (1): 65-70.
  • Yoshizaki A, et al. (2006) Expression of interleukin (IL)-11 and IL-11 receptor in human colorectal adenocarcinoma: IL-11 up-regulation of the invasive and proliferative activity of human colorectal carcinoma cells. Int J Oncol. 29 (4): 869-76.
  • Karube K, et al. (2006) Gene expression profile of cytokines and chemokines in microdissected primary Hodgkin and Reed-Sternberg (HRS) cells: high expression of interleukin-11 receptor alpha. Ann Oncol. 17 (1): 110-6.
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    Cat No: HG10252-M-F
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