휴먼(Human) IL20Ra 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10397-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_014432.2

cDNA Size:   1662

Synonym:  IL-20R1, ZCYTOR7, FLJ40993, IL20RA

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens interleukin 20 receptor, alpha (IL20RA) DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for a point mutation 775G/A resulting in the encoded amino acid 259Val replacement by Ile.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGCGGGCTC CCGGCCGCCC GGCCCTGCGG CCGCTGCCGC TGCCGCCGCT GCTGCTGTTG
61       CTCCTGGCGG CGCCTTGGGG ACGGGCAGTT CCCTGTGTCT CTGGTGGTTT GCCTAAACCT
121      GCAAACATCA CCTTCTTATC CATCAACATG AAGAATGTCC TACAATGGAC TCCACCAGAG
181      GGTCTTCAAG GAGTTAAAGT TACTTACACT GTGCAGTATT TCATATATGG GCAAAAGAAA
241      TGGCTGAATA AATCAGAATG CAGAAATATC AATAGAACCT ACTGTGATCT TTCTGCTGAA
301      ACTTCTGACT ACGAACACCA GTATTATGCC AAAGTTAAGG CCATTTGGGG AACAAAGTGT
361      TCCAAATGGG CTGAAAGTGG ACGGTTCTAT CCTTTTTTAG AAACACAAAT TGGCCCACCA
421      GAGGTGGCAC TGACTACAGA TGAGAAGTCC ATTTCTGTTG TCCTGACAGC TCCAGAGAAG
481      TGGAAGAGAA ATCCAGAAGA CCTTCCTGTT TCCATGCAAC AAATATACTC CAATCTGAAG
541      TATAACGTGT CTGTGTTGAA TACTAAATCA AACAGAACGT GGTCCCAGTG TGTGACCAAC
601      CACACGCTGG TGCTCACCTG GCTGGAGCCG AACACTCTTT ACTGCGTACA CGTGGAGTCC
661      TTCGTCCCAG GGCCCCCTCG CCGTGCTCAG CCTTCTGAGA AGCAGTGTGC CAGGACTTTG
721      AAAGATCAAT CATCAGAGTT CAAGGCTAAA ATCATCTTCT GGTATGTTTT GCCCATATCT
781      ATTACCGTGT TTCTTTTTTC TGTGATGGGC TATTCCATCT ACCGATATAT CCACGTTGGC
841      AAAGAGAAAC ACCCAGCAAA TTTGATTTTG ATTTATGGAA ATGAATTTGA CAAAAGATTC
901      TTTGTGCCTG CTGAAAAAAT CGTGATTAAC TTTATCACCC TCAATATCTC GGATGATTCT
961      AAAATTTCTC ATCAGGATAT GAGTTTACTG GGAAAAAGCA GTGATGTATC CAGCCTTAAT
1021     GATCCTCAGC CCAGCGGGAA CCTGAGGCCC CCTCAGGAGG AAGAGGAGGT GAAACATTTA
1081     GGGTATGCTT CGCATTTGAT GGAAATTTTT TGTGACTCTG AAGAAAACAC GGAAGGTACT
1141     TCTCTCACCC AGCAAGAGTC CCTCAGCAGA ACAATACCCC CGGATAAAAC AGTCATTGAA
1201     TATGAATATG ATGTCAGAAC CACTGACATT TGTGCGGGGC CTGAAGAGCA GGAGCTCAGT
1261     TTGCAGGAGG AGGTGTCCAC ACAAGGAACA TTATTGGAGT CGCAGGCAGC GTTGGCAGTC
1321     TTGGGCCCGC AAACGTTACA GTACTCATAC ACCCCTCAGC TCCAAGACTT AGACCCCCTG
1381     GCGCAGGAGC ACACAGACTC GGAGGAGGGG CCGGAGGAAG AGCCATCGAC GACCCTGGTC
1441     GACTGGGATC CCCAAACTGG CAGGCTGTGT ATTCCTTCGC TGTCCAGCTT CGACCAGGAT
1501     TCAGAGGGCT GCGAGCCTTC TGAGGGGGAT GGGCTCGGAG AGGAGGGTCT TCTATCTAGA
1561     CTCTATGAGG AGCCGGCTCC AGACAGGCCA CCAGGAGAAA ATGAAACCTA TCTCATGCAA
1621     TTCATGGAGG AATGGGGGTT ATATGTGCAG ATGGAAAACT GA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) IL20Ra 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)