인플루엔자(Influenza) B (B/Malaysia/2506/2004) 적혈구응집성(Hemagglutinin)

Influenza B ( B/Malaysia/2506/2004 ) HA Protein

Influenza B ( B/Malaysia/2506/2004 ) HA Protein Product Information

• Synonym: HA
• Protein Construction: A DNA sequence encoding the the influenza hemagglutinin ( B/Malaysia/2506/2004 ) (ACO05957.1) ( Met 1 - Ala 556 )( HA1+HA2, uncleaved ), was fused with a C-terminal polyhistidine tag
• Source: Influenza B Virus
• Expression Host: Human Cells

Influenza B ( B/Malaysia/2506/2004 ) HA Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 16
• Molecular Mass: The secreted recombinant influenza B HA (B/Malaysia/2506/2004) comprises 552 amino acids and has a predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE: Influenza B HA protein

Influenza B ( B/Malaysia/2506/2004 ) HA Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Related Influenza Virus Research Tools

• Influenza Virus Research Tools
• Influenza Hemagglutinin & Antibody
• Influenza Hemagglutinin Proteins
• Influenza B Hemagglutinin & Antibody
• B/Malaysia/2506/2004

Influenza B ( B/Malaysia/2506/2004 ) HA Protein Description

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species. Influenza B is largely confined to humans and is an important cause of morbidity. Influenza C infects humans, dogs and pigs, sometimes causing both severe illness and local epidemics. Influenza B viruses are only known to infect humans and seals, giving them influenza. Influenza B viruses evolve slower than A viruses and faster than C viruses. Influenza B virus mutates at a rate 2 to 3 times lower than type A. However, influenza B mutates enough that lasting immunity is not possible. Hemagglutinin (HA) is a Single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. It is a homotrimer of disulfide-linked HA1-HA2. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

References

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4. Taubenberger JK, et al., 2008. Annu Rev Pathol. 3: 499-522.
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6. Guan Y, et al., 2009, Revue scientifique et technique. 23 (2): 453-65.