랫트(Rat) KIM1/TIM1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

HAVCR1 cDNA Clone Datasheet

HAVCR1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_173149.1
• cDNA Size: 924
• Gene Synonym: Kim1; KIM-1; Havcr1
• Source: Rat

HAVCR1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus hepatitis A virus cellular receptor 1 DNA.
• Vector: pMD18-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

HAVCR1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HAVCR1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HAVCR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTTCAAC TTCAAGTCTT CATTTCAGGC CTCCTGCTGC TTCTTCCAGG CTCTGTAGAT
61       TCTTATGAAG TAGTGAAGGG GGTGGTGGGT CACCCTGTCA CAATTCCATG TACTTACTCA
121      ACACGTGGAG GAATCACAAC GACATGTTGG GGCCGGGGGC AATGCCCATA TTCTAGTTGT
181      CAAAATATAC TTATTTGGAC CAATGGATAC CAAGTCACCT ATCGGAGCAG CGGTCGATAC
241      AACATAAAGG GGCGTATTTC AGAAGGAGAC GTATCCTTGA CAATAGAGAA CTCTGTTGAT
301      AGTGATAGTG GTCTGTATTG TTGCCGAGTG GAGATTCCTG GATGGTTCAA CGATCAGAAA
361      ATGACCTTTT CATTGGAAGT TAAACCAGAA ATTCCCACAA GTCCTCCAAC AAGACCCACA
421      ACTACAAGAC CCACAACCAC AAGGCCCACA ACTATTTCAA CAAGATCCAC ACATGTACCA
481      ACATCAACCA GAGTCTCCAC CTCTACTCCA ACACCAGAAC AAACACAGAC TCACAAACCA
541      GAAATCACTA CATTTTATGC CCATGAGACA ACTGCTGAGG TGACAGAAAC TCCATCATAT
601      ACTCCTGCAG ACTGGAATGG CACTGTGACA TCCTCAGAGG AGGCCTGGAA TAATCACACT
661      GTAAGAATCC CTTTGAGGAA GCCGCAGAGA AACCCGACTA AGGGCTTCTA TGTTGGCATG
721      TCCGTTGCAG CCCTGCTGCT GCTGCTGCTT GCGAGCACCG TGGTTGTCAC CAGGTACATC
781      ATTATAAGAA AGAAGATGGG CTCTCTGAGC TTTGTTGCCT TCCATGTCTC TAAGAGTAGA
841      GCTTTGCAGA ACGCAGCGAT TGTGCATCCC CGAGCTGAAG ACAACATCTA CATTATTGAA
901      GATAGATCTC GAGGTGCAGA ATGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).