휴먼(Human) LCN1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

LCN1 cDNA Clone Datasheet

LCN1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002297.2
• cDNA Size: 531
• Gene Synonym: TP; PMFA; VEGP; MGC71975; LCN1
• Source: Human

LCN1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens lipocalin 1 (tear prealbumin) DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

LCN1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LCN1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

LCN1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGCCCC TGCTCCTGGC CGTCAGCCTT GGCCTCATTG CTGCCCTGCA GGCCCACCAC
61       CTCCTGGCCT CAGACGAGGA GATTCAGGAT GTGTCAGGGA CGTGGTATCT GAAGGCCATG
121      ACGGTGGACA GGGAGTTCCC TGAGATGAAT CTGGAATCGG TGACACCCAT GACCCTCACG
181      ACCCTGGAAG GGGGCAACCT GGAAGCCAAG GTCACCATGC TGATAAGTGG CCGGTGCCAG
241      GAGGTGAAGG CCGTCCTGGA GAAAACTGAC GAGCCGGGAA AATACACGGC CGACGGGGGC
301      AAGCACGTGG CATACATCAT CAGGTCGCAC GTGAAGGACC ACTACATCTT TTACTGTGAG
361      GGCGAGCTGC ACGGGAAGCC GGTCCGAGGG GTGAAGCTCG TGGGCAGAGA CCCCAAGAAC
421      AACCTGGAAG CCTTGGAGGA CTTTGAGAAA GCCGCAGGAG CCCGCGGACT CAGCACGGAG
481      AGCATCCTCA TCCCCAGGCA GAGCGAAACC TGCTCTCCAG GGAGCGATTA G

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).