휴먼(Human) LRRN3 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

LRRN3 cDNA Clone Datasheet

LRRN3 cDNA Clone Product Information

• Gene Bank Ref.ID: BC035133.2
• cDNA Size: 2127
• Gene Synonym: NLRR3; NLRR-3; FIGLER5; FLJ11129; LRRN3
• Source: Human

LRRN3 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens leucine rich repeat neuronal 3 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

LRRN3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LRRN3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

LRRN3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGGACA TGCCACTCCG AATTCATGTG CTACTTGGCC TAGCTATCAC TACACTAGTA
61       CAAGCTGTAG ATAAAAAAGT GGATTGTCCA CGGTTATGTA CGTGTGAAAT CAGGCCTTGG
121      TTTACACCCA GATCCATTTA TATGGAAGCA TCTACAGTGG ATTGTAATGA TTTAGGTCTT
181      TTAACTTTCC CAGCCAGATT GCCAGCTAAC ACACAGATTC TTCTCCTACA GACTAACAAT
241      ATTGCAAAAA TTGAATACTC CACAGACTTT CCAGTAAACC TTACTAGCCT GGATTTATCT
301      CAAAACAATT TATCTTCAGT CACCAATATT AATGTAAAAA AGATGCCTCA GCTCCTTTCT
361      GTGTACCTAG AGGAAAACAA ACTTACTGAA CTGCCTGAAA AATGTCTGTC CGAACTGAGC
421      AACTTACAAG AACTCTATAT TAATCACAAC TTGCTTTCTA CAATTTCACC TGGAGCCTTT
481      ATTGGCCTAC ATAATCTTCT TCGACTTCAT CTCAATTCAA ATAGATTGCA GATGATCAAC
541      AGTAAGTGGT TTGATGCTCT TCCAAATCTA GAGATTCTGA TGATTGGGGA AAATCCAATT
601      ATCAGAATCA AAGACATGAA CTTTAAGCCT CTTATCAATC TTCGCAGCCT GGTTATAGCT
661      GGTATAAACC TCACAGAAAT ACCAGATAAC GCCTTGGTTG GACTGGAAAA CTTAGAAAGC
721      ATCTCTTTTT ACGATAACAG GCTTATTAAA GTACCCCATG TTGCTCTTCA AAAAGTTGTA
781      AATCTCAAAT TTTTGGATCT AAATAAAAAT CCTATTAATA GAATACGAAG GGGTGATTTT
841      AGCAATATGC TACACTTAAA AGAGTTGGGG ATAAATAATA TGCCTGAGCT GATTTCCATC
901      GATAGTCTTG CTGTGGATAA CCTGCCAGAT TTAAGAAAAA TAGAAGCTAC TAACAACCCT
961      AGATTGTCTT ACATTCACCC CAATGCATTT TTCAGACTCC CCAAGCTGGA ATCACTCATG
1021     CTGAACAGCA ATGCTCTCAG TGCCCTGTAC CATGGTACCA TTGAGTCTCT GCCAAACCTC
1081     AAGGAAATCA GCATACACAG TAACCCCATC AGGTGTGACT GTGTCATCCG TTGGATGAAC
1141     ATGAACAAAA CCAACATTCG ATTCATGGAG CCAGATTCAC TGTTTTGCGT GGACCCACCT
1201     GAATTCCAAG GTCAGAATGT TCGGCAAGTG CATTTCAGGG ACATGATGGA AATTTGTCTC
1261     CCTCTTATAG CTCCTGAGAG CTTTCCTTCT AATCTAAATG TAGAAGCTGG GAGCTATGTT
1321     TCCTTTCACT GTAGAGCTAC TGCAGAACCA CAGCCTGAAA TCTACTGGAT AACACCTTCT
1381     GGTCAAAAAC TCTTGCCTAA TACCCTGACA GACAAGTTCT ATGTCCATTC TGAGGGAACA
1441     CTAGATATAA ATGGCGTAAC TCCCAAAGAA GGGGGTTTAT ATACTTGTAT AGCAACTAAC
1501     CTAGTTGGCG CTGACTTGAA GTCTGTTATG ATCAAAGTGG ATGGATCTTT TCCACAAGAT
1561     AACAATGGCT CTTTGAATAT TAAAATAAGA GATATTCATG CCAATTCAGT TTTGGTGTCC
1621     TGGAAAGCAA GTTCTAAAAT TCTCAAATCT AGTGTTAAAT GGACAGCCTT TGTCAAGACT
1681     GAAAATTCTC ATGCTGCGCA AAGTGCTCGA ATACCATCTG ATGTCAAGGT ATATAATCTT
1741     ACTCATCTGA ATCCATCAAC TGAGTATAAA ATTTGTATTG ATATTCCCAC CATCTATCAG
1801     AAAAACAGAA AAAAATGTGT AAATGTCACC ACCAAAGGTT TGCACCCTGA TCAAAAAGAG
1861     TATGAAAAGA ATAATACCAC AACACTTATG GCCTGTCTTG GAGGCCTTCT GGGGATTATT
1921     GGTGTGATAT GTCTTATCAG CTGCCTCTCT CCAGAAATGA ACTGTGATGG TGGACACAGC
1981     TATGTGAGGA ATTACTTACA GAAACCAACC TTTGCATTAG GTGAGCTTTA TCCTCCTCTG
2041     ATAAATCTCT GGGAAGCAGG AAAAGAAAAA AGTACATCAC TGAAAGTAAA AGCAACTGTT
2101     ATAGGTTTAC CAACAAATAT GTCCTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).