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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) LTA4H 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) LTA4H 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10276-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_000895.1
cDNA Size: 1836
Synonym: LTA4H
Source: Human.
cDNA Description: ORF Clone of Homo sapiens leukotriene A4 hydrolase DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for a point mutation1732T/G causing the 578Phe to be replaced by Val.
10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGCCCGAGA TAGTGGATAC CTGTTCGTTG GCCTCTCCGG CTTCCGTCTG CCGGACCAAG 61 CACCTGCACC TGCGCTGCAG CGTCGACTTT ACTCGCCGGA CGCTGACCGG GACTGCTGCT 121 CTCACGGTCC AGTCTCAGGA GGACAATCTG CGCAGCCTGG TTTTGGATAC AAAGGACCTT 181 ACAATAGAAA AAGTAGTGAT CAATGGACAA GAAGTCAAAT ATGCTCTTGG AGAAAGACAA 241 AGTTACAAGG GATCGCCAAT GGAAATCTCT CTTCCTATCG CTTTGAGCAA AAATCAAGAA 301 ATTGTTATAG AAATTTCTTT TGAGACCTCT CCAAAATCTT CTGCTCTCCA GTGGCTCACT 361 CCTGAACAGA CTTCTGGGAA GGAACACCCA TATCTCTTTA GTCAGTGCCA GGCCATCCAC 421 TGCAGAGCAA TCCTTCCTTG TCAGGACACT CCTTCTGTGA AATTAACCTA TACTGCAGAG 481 GTGTCTGTCC CTAAAGAACT GGTGGCACTT ATGAGTGCTA TTCGTGATGG AGAAACACCT 541 GACCCAGAAG ACCCAAGCAG GAAAATATAC AAATTCATCC AAAAAGTTCC AATACCCTGC 601 TACCTGATTG CTTTAGTTGT TGGAGCTTTA GAAAGCAGGC AAATTGGCCC AAGAACTTTG 661 GTGTGGTCTG AGAAAGAGCA GGTGGAAAAG TCTGCTTATG AGTTTTCTGA GACTGAATCT 721 ATGCTTAAAA TAGCAGAAGA TCTGGGAGGA CCGTATGTAT GGGGACAGTA TGACCTATTG 781 GTCCTGCCAC CATCCTTCCC TTATGGTGGC ATGGAGAATC CTTGCCTTAC TTTTGTAACT 841 CCTACTCTAC TGGCAGGCGA CAAGTCACTC TCCAATGTCA TTGCACATGA AATATCTCAT 901 AGCTGGACAG GGAATCTAGT GACCAACAAA ACTTGGGATC ACTTTTGGTT AAATGAGGGA 961 CATACTGTGT ACTTGGAACG CCACATTTGC GGACGATTGT TTGGTGAAAA GTTCAGACAT 1021 TTTAATGCTC TGGGAGGATG GGGAGAACTA CAGAATTCGG TAAAGACATT TGGGGAGACA 1081 CATCCTTTCA CCAAACTTGT GGTTGATCTG ACAGATATAG ACCCTGATGT AGCTTATTCT 1141 TCAGTTCCCT ATGAGAAGGG CTTTGCTTTA CTTTTTTACC TTGAACAACT GCTTGGAGGA 1201 CCAGAGATTT TCCTAGGATT CTTAAAAGCT TATGTTGAGA AGTTTTCCTA TAAGAGCATA 1261 ACTACTGATG ACTGGAAGGA TTTCCTGTAT TCCTATTTTA AAGATAAGGT TGATGTTCTC 1321 AATCAAGTTG ATTGGAATGC CTGGCTCTAC TCTCCTGGAC TGCCTCCCAT AAAGCCCAAT 1381 TATGATATGA CTCTGACAAA TGCTTGTATT GCCTTAAGTC AAAGATGGAT TACTGCCAAA 1441 GAAGATGATT TAAATTCATT CAATGCCACA GACCTGAAGG ATCTCTCTTC TCATCAATTG 1501 AATGAGTTTT TAGCACAGAC GCTCCAGAGG GCACCTCTTC CATTGGGGCA CATAAAGCGA 1561 ATGCAAGAGG TGTACAACTT CAATGCCATT AACAATTCTG AAATACGATT CAGATGGCTG 1621 CGGCTCTGCA TTCAATCCAA GTGGGAGGAC GCAATTCCTT TGGCGCTAAA GATGGCAACT 1681 GAACAAGGAA GAATGAAGTT TACCCGGCCC TTATTCAAGG ATCTTGCTGC CGTTGACAAA 1741 TCCCATGATC AAGCTGTCCG AACCTACCAA GAGCACAAAG CAAGCATGCA TCCCGTGACT 1801 GCAATGCTGG TGGGGAAAGA CTTAAAAGTG GATTAA
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
