MMP-3 Protein, Human, Recombinant

1/1
Price:
Size:
Number:

MMP-3 Protein, Human, Recombinant: Product Information

Purity
> 97 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nva-WR-K(Dnp)-NH2, AnaSpec, Catalog # 27114.
The specific activity is >300 pmoles/min/μg.
(Activation description: The proenzyme needs to be activated by Chymotrypsin for an activated form)
Protein Construction
A DNA sequence encoding the human MMP3 (AAA36321.1) N-terminal fragment (Tyr 18-Thr 272) was expressed and purified.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met 1
Molecule Mass
The recombinant human MMP3 consisting of 256 amino acids and has a calculated molecular mass of 29 KDa. It migrates as an approximately 34 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 50mM Tris, 10mM CaCL2, 1uM ZnCL2, 50mM NaCl, 0.5% Brij35, pH 7.0
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MMP-3 Protein, Human, Recombinant: Images

MMP-3 Protein, Human, Recombinant: Alternative Names

CHDS6 Protein, Human; MMP-3 Protein, Human; SL-1 Protein, Human; STMY Protein, Human; STMY1 Protein, Human; STR1 Protein, Human

MMP-3 Background Information

Matrix metallopeptidase 3 (abbreviated as MMP3) is also known as stromelysin 1 and progelatinase. MMP3 is a member of the matrix metalloproteinase (MMP) family whose members are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes including arthritis and metastasis. As a secreted zinc-dependent endopeptidase, MMP3 exerts its functions mainly in extracellular matrix. This protein is activated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMP3 plays a central role in degrading collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP3 can also active other MMPs such as MMP1, MMP7, and MMP9, rendering MMP3 crucial in connective tissue remodeling. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Synthetic or natural inhibitors of MMPs result in inhibition of metastasis, while up-regulation of MMPs led to enhanced cancer cell invasion.
Full Name
matrix metallopeptidase 3
References
  • Sternlicht MD, et al. 1999, The stromal proteinase MMP3/stromelysin-1 promotes mammary carcinogenesis. Cell. 98(2): 137-46.
  • Yoon S, et al. (1999) Genetic analysis of MMP3, MMP9, and PAI-1 in Finnish patients with abdominal aortic or intracranial aneurysms. Biochem Biophys Res Commun. 265(2): 563-8.
  • Biondi ML, et al. (2000) MMP1 and MMP3 polymorphisms in promoter regions and cancer. Clin Chem. 46(12): 2023-4.
  • Peptide Microarray-Based Metal Enhanced Fluorescence Assay for Multiple Profiling of Matrix Metalloproteinases Activities
    Author
    Lei, Z;Zhang, H;Wang, Y;Meng, X;Wang, Z;
    Year
    2017
    Journal
    Anal. Chem.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait