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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) MMP-9 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) MMP-9 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10327-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_004994.2
cDNA Size: 2124
Synonym: GELB, CLG4B, MMP-9
Source: Human.
cDNA Description: ORF Clone of Homo sapiens matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) DNA.
Vector: Vector pMD18-T Simple
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 836 A/G resulting in the amino acid Gln substitution by Arg, 1721 G/C resulting in the amino acid Arg substitution by Pro, 2003 G/A resulting in the amino acid Arg substitution by Gln, and 2082 G/A not causing the amino acid variation.
5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
1 ATGAGCCTCT GGCAGCCCCT GGTCCTGGTG CTCCTGGTGC TGGGCTGCTG CTTTGCTGCC 61 CCCAGACAGC GCCAGTCCAC CCTTGTGCTC TTCCCTGGAG ACCTGAGAAC CAATCTCACC 121 GACAGGCAGC TGGCAGAGGA ATACCTGTAC CGCTATGGTT ACACTCGGGT GGCAGAGATG 181 CGTGGAGAGT CGAAATCTCT GGGGCCTGCG CTGCTGCTTC TCCAGAAGCA ACTGTCCCTG 241 CCCGAGACCG GTGAGCTGGA TAGCGCCACG CTGAAGGCCA TGCGAACCCC ACGGTGCGGG 301 GTCCCAGACC TGGGCAGATT CCAAACCTTT GAGGGCGACC TCAAGTGGCA CCACCACAAC 361 ATCACCTATT GGATCCAAAA CTACTCGGAA GACTTGCCGC GGGCGGTGAT TGACGACGCC 421 TTTGCCCGCG CCTTCGCACT GTGGAGCGCG GTGACGCCGC TCACCTTCAC TCGCGTGTAC 481 AGCCGGGACG CAGACATCGT CATCCAGTTT GGTGTCGCGG AGCACGGAGA CGGGTATCCC 541 TTCGACGGGA AGGACGGGCT CCTGGCACAC GCCTTTCCTC CTGGCCCCGG CATTCAGGGA 601 GACGCCCATT TCGACGATGA CGAGTTGTGG TCCCTGGGCA AGGGCGTCGT GGTTCCAACT 661 CGGTTTGGAA ACGCAGATGG CGCGGCCTGC CACTTCCCCT TCATCTTCGA GGGCCGCTCC 721 TACTCTGCCT GCACCACCGA CGGTCGCTCC GACGGCTTGC CCTGGTGCAG TACCACGGCC 781 AACTACGACA CCGACGACCG GTTTGGCTTC TGCCCCAGCG AGAGACTCTA CACCCAGGAC 841 GGCAATGCTG ATGGGAAACC CTGCCAGTTT CCATTCATCT TCCAAGGCCA ATCCTACTCC 901 GCCTGCACCA CGGACGGTCG CTCCGACGGC TACCGCTGGT GCGCCACCAC CGCCAACTAC 961 GACCGGGACA AGCTCTTCGG CTTCTGCCCG ACCCGAGCTG ACTCGACGGT GATGGGGGGC 1021 AACTCGGCGG GGGAGCTGTG CGTCTTCCCC TTCACTTTCC TGGGTAAGGA GTACTCGACC 1081 TGTACCAGCG AGGGCCGCGG AGATGGGCGC CTCTGGTGCG CTACCACCTC GAACTTTGAC 1141 AGCGACAAGA AGTGGGGCTT CTGCCCGGAC CAAGGATACA GTTTGTTCCT CGTGGCGGCG 1201 CATGAGTTCG GCCACGCGCT GGGCTTAGAT CATTCCTCAG TGCCGGAGGC GCTCATGTAC 1261 CCTATGTACC GCTTCACTGA GGGGCCCCCC TTGCATAAGG ACGACGTGAA TGGCATCCGG 1321 CACCTCTATG GTCCTCGCCC TGAACCTGAG CCACGGCCTC CAACCACCAC CACACCGCAG 1381 CCCACGGCTC CCCCGACGGT CTGCCCCACC GGACCCCCCA CTGTCCACCC CTCAGAGCGC 1441 CCCACAGCTG GCCCCACAGG TCCCCCCTCA GCTGGCCCCA CAGGTCCCCC CACTGCTGGC 1501 CCTTCTACGG CCACTACTGT GCCTTTGAGT CCGGTGGACG ATGCCTGCAA CGTGAACATC 1561 TTCGACGCCA TCGCGGAGAT TGGGAACCAG CTGTATTTGT TCAAGGATGG GAAGTACTGG 1621 CGATTCTCTG AGGGCAGGGG GAGCCGGCCG CAGGGCCCCT TCCTTATCGC CGACAAGTGG 1681 CCCGCGCTGC CCCGCAAGCT GGACTCGGTC TTTGAGGAGC GGCTCTCCAA GAAGCTTTTC 1741 TTCTTCTCTG GGCGCCAGGT GTGGGTGTAC ACAGGCGCGT CGGTGCTGGG CCCGAGGCGT 1801 CTGGACAAGC TGGGCCTGGG AGCCGACGTG GCCCAGGTGA CCGGGGCCCT CCGGAGTGGC 1861 AGGGGGAAGA TGCTGCTGTT CAGCGGGCGG CGCCTCTGGA GGTTCGACGT GAAGGCGCAG 1921 ATGGTGGATC CCCGGAGCGC CAGCGAGGTG GACCGGATGT TCCCCGGGGT GCCTTTGGAC 1981 ACGCACGACG TCTTCCAGTA CCGAGAGAAA GCCTATTTCT GCCAGGACCG CTTCTACTGG 2041 CGCGTGAGTT CCCGGAGTGA GTTGAACCAG GTGGACCAAG TAGGCTACGT GACCTATGAC 2101 ATCCTGCAGT GCCCTGAGGA CTAG
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
