휴먼(Human) MSP 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10406-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_020998.3

cDNA Size:   2136

Synonym:  MSP, HGFL, NF15S2, D3F15S2, DNF15S2, MST1

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens macrophage stimulating 1(hepatocyte growth factor-like) DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the point mutations: 417A/G and 936G/T, neither of which results in amino acid variation.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGGGTGGC TCCCACTCCT GCTGCTTCTG ACTCAATGCT TAGGGGTCCC TGGGCAGCGC
61       TCGCCATTGA ATGACTTCCA AGTGCTCCGG GGCACAGAGC TACAGCACCT GCTACATGCG
121      GTGGTGCCCG GGCCTTGGCA GGAGGATGTG GCAGATGCTG AAGAGTGTGC TGGTCGCTGT
181      GGGCCCTTAA TGGACTGCCG GGCCTTCCAC TACAACGTGA GCAGCCATGG TTGCCAACTG
241      CTGCCATGGA CTCAACACTC GCCCCACACG AGGCTGCGGC GTTCTGGGCG CTGTGACCTC
301      TTCCAGAAGA AAGACTACGT ACGGACCTGC ATCATGAACA ATGGGGTTGG GTACCGGGGC
361      ACCATGGCCA CGACCGTGGG TGGCCTGCCC TGCCAGGCTT GGAGCCACAA GTTCCCGAAT
421      GATCACAAGT ACACGCCCAC TCTCCGGAAT GGCCTGGAAG AGAACTTCTG CCGTAACCCT
481      GATGGCGACC CCGGAGGTCC TTGGTGCTAC ACAACAGACC CTGCTGTGCG CTTCCAGAGC
541      TGCGGCATCA AATCCTGCCG GGAGGCCGCG TGTGTCTGGT GCAATGGCGA GGAATACCGC
601      GGCGCGGTAG ACCGCACGGA GTCAGGGCGC GAGTGCCAGC GCTGGGATCT TCAGCACCCG
661      CACCAGCACC CCTTCGAGCC GGGCAAGTTC CTCGACCAAG GTCTGGACGA CAACTATTGC
721      CGGAATCCTG ACGGCTCCGA GCGGCCATGG TGCTACACTA CGGATCCGCA GATCGAGCGA
781      GAGTTCTGTG ACCTCCCCCG CTGCGGGTCC GAGGCACAGC CCCGCCAAGA GGCCACAACT
841      GTCAGCTGCT TCCGCGGGAA GGGTGAGGGC TACCGGGGCA CAGCCAATAC CACCACTGCG
901      GGCGTACCTT GCCAGCGTTG GGACGCGCAA ATCCCTCATC AGCACCGATT TACGCCAGAA
961      AAATACGCGT GCAAAGACCT TCGGGAGAAC TTCTGCCGGA ACCCCGACGG CTCAGAGGCG
1021     CCCTGGTGCT TCACACTGCG GCCCGGCATG CGCGCGGCCT TTTGCTACCA GATCCGGCGT
1081     TGTACAGACG ACGTGCGGCC CCAGGACTGC TACCACGGCG CAGGGGAGCA GTACCGCGGC
1141     ACGGTCAGCA AGACCCGCAA GGGTGTCCAG TGCCAGCGCT GGTCCGCTGA GACGCCGCAC
1201     AAGCCGCAGT TCACGTTTAC CTCCGAACCG CATGCACAAC TGGAGGAGAA CTTCTGCCGG
1261     AACCCAGATG GGGATAGCCA TGGGCCCTGG TGCTACACGA TGGACCCAAG GACCCCATTC
1321     GACTACTGTG CCCTGCGACG CTGCGCTGAT GACCAGCCGC CATCAATCCT GGACCCCCCA
1381     GACCAGGTGC AGTTTGAGAA GTGTGGCAAG AGGGTGGATC GGCTGGATCA GCGGCGTTCC
1441     AAGCTGCGCG TGGTTGGGGG CCATCCGGGC AACTCACCCT GGACAGTCAG CTTGCGGAAT
1501     CGGCAGGGCC AGCATTTCTG CGGGGGGTCT CTAGTGAAGG AGCAGTGGAT ACTGACTGCC
1561     CGGCAGTGCT TCTCCTCCTG CCATATGCCT CTCACGGGCT ATGAGGTATG GTTGGGCACC
1621     CTGTTCCAGA ACCCACAGCA TGGAGAGCCA AGCCTACAGC GGGTCCCAGT AGCCAAGATG
1681     GTGTGTGGGC CCTCAGGCTC CCAGCTTGTC CTGCTCAAGC TGGAGAGATC TGTGACCCTG
1741     AACCAGCGTG TGGCCCTGAT CTGCCTGCCC CCTGAATGGT ATGTGGTGCC TCCAGGGACC
1801     AAGTGTGAGA TTGCAGGCTG GGGTGAGACC AAAGGTACGG GTAATGACAC AGTCCTAAAT
1861     GTGGCCTTGC TGAATGTCAT CTCCAACCAG GAGTGTAACA TCAAGCACCG AGGACGTGTG
1921     CGGGAGAGTG AGATGTGCAC TGAGGGACTG TTGGCCCCTG TGGGGGCCTG TGAGGGTGAC
1981     TACGGGGGCC CACTTGCCTG CTTTACCCAC AACTGCTGGG TCCTGGAAGG AATTATAATC
2041     CCCAACCGAG TATGCGCAAG GTCCCGCTGG CCAGCTGTCT TCACGCGTGT CTCTGTGTTT
2101     GTGGACTGGA TTCACAAGGT CATGAGACTG GGTTAG

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) MSP 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)