휴먼(Human) MST1R 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

MST1R cDNA Clone Datasheet

MST1R cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002447.2
• cDNA Size: 4203
• Gene Synonym: RON; PTK8; CD136; CDw136; MST1R
• Source: Human

MST1R cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens macrophage stimulating 1 receptor (c-met-related tyrosine kinase) DNA.
• Vector: pMD18-T Vector
• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide (2648-2767) deletion mutation and the point mutation 3583 A/G which results in the amino acid Ser replacement by Gly.

MST1R cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MST1R cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

MST1R cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCTCC TCCCGCCGCT GCCTCAGTCC TTCCTGTTGC TGCTGCTGTT GCCTGCCAAG
61       CCCGCGGCGG GCGAGGACTG GCAGTGCCCG CGCACCCCCT ACGCGGCCTC TCGCGACTTT
121      GACGTGAAGT ACGTGGTGCC CAGCTTCTCC GCCGGAGGCC TGGTACAGGC CATGGTGACC
181      TACGAGGGCG ACAGAAATGA GAGTGCTGTG TTTGTAGCCA TACGCAATCG CCTGCATGTG
241      CTTGGGCCTG ACCTGAAGTC TGTCCAGAGC CTGGCCACGG GCCCTGCTGG AGACCCTGGC
301      TGCCAGACGT GTGCAGCCTG TGGCCCAGGA CCCCACGGCC CTCCCGGTGA CACAGACACA
361      AAGGTGCTGG TGCTGGATCC CGCGCTGCCT GCGCTGGTCA GTTGTGGCTC CAGCCTGCAG
421      GGCCGCTGCT TCCTGCATGA CCTAGAGCCC CAAGGGACAG CCGTGCATCT GGCAGCGCCA
481      GCCTGCCTCT TCTCAGCCCA CCATAACCGG CCCGATGACT GCCCCGACTG TGTGGCCAGC
541      CCATTGGGCA CCCGTGTAAC TGTGGTTGAG CAAGGCCAGG CCTCCTATTT CTACGTGGCA
601      TCCTCACTGG ACGCAGCCGT GGCTGCCAGC TTCAGCCCAC GCTCAGTGTC TATCAGGCGT
661      CTCAAGGCTG ACGCCTCGGG ATTCGCACCG GGCTTTGTGG CGTTGTCAGT GCTGCCCAAG
721      CATCTTGTCT CCTACAGTAT TGAATACGTG CACAGCTTCC ACACGGGAGC CTTCGTATAC
781      TTCCTGACTG TACAGCCGGC CAGCGTGACA GATGATCCTA GTGCCCTGCA CACACGCCTG
841      GCACGGCTTA GCGCCACTGA GCCAGAGTTG GGTGACTATC GGGAGCTGGT CCTCGACTGC
901      AGATTTGCTC CAAAACGCAG GCGCCGGGGG GCCCCAGAAG GCGGACAGCC CTACCCTGTG
961      CTGCGGGTGG CCCACTCCGC TCCAGTGGGT GCCCAACTTG CCACTGAGCT GAGCATCGCC
1021     GAGGGCCAGG AAGTACTATT TGGGGTCTTT GTGACTGGCA AGGATGGTGG TCCTGGCGTG
1081     GGCCCCAACT CTGTCGTCTG TGCCTTCCCC ATTGACCTGC TGGACACACT AATTGATGAG
1141     GGTGTGGAGC GCTGTTGTGA ATCCCCAGTC CATCCAGGCC TCCGGCGAGG CCTCGACTTC
1201     TTCCAGTCGC CCAGTTTTTG CCCCAACCCG CCTGGCCTGG AAGCCCTCAG CCCCAACACC
1261     AGCTGCCGCC ACTTCCCTCT GCTGGTCAGT AGCAGCTTCT CACGTGTGGA CCTATTCAAT
1321     GGGCTGTTGG GACCAGTACA GGTCACTGCA TTGTATGTGA CACGCCTTGA CAACGTCACA
1381     GTGGCACACA TGGGCACAAT GGATGGGCGT ATCCTGCAGG TGGAGCTGGT CAGGTCACTA
1441     AACTACTTGC TGTATGTGTC CAACTTCTCA CTGGGTGACA GTGGGCAGCC CGTGCAGCGG
1501     GATGTCAGTC GTCTTGGGGA CCACCTACTC TTTGCCTCTG GGGACCAGGT TTTCCAGGTA
1561     CCTATCCAAG GCCCTGGCTG CCGCCACTTC CTGACCTGTG GGCGTTGCCT AAGGGCATGG
1621     CATTTCATGG GCTGTGGCTG GTGTGGGAAC ATGTGCGGCC AGCAGAAGGA GTGTCCTGGC
1681     TCCTGGCAAC AGGACCACTG CCCACCTAAG CTTACTGAGT TCCACCCCCA CAGTGGACCT
1741     CTAAGGGGCA GTACAAGGCT GACCCTGTGT GGCTCCAACT TCTACCTTCA CCCTTCTGGT
1801     CTGGTGCCTG AGGGAACCCA TCAGGTCACT GTGGGCCAAA GTCCCTGCCG GCCACTGCCC
1861     AAGGACAGCT CAAAACTCAG ACCAGTGCCC CGGAAAGACT TTGTAGAGGA GTTTGAGTGT
1921     GAACTGGAGC CCTTGGGCAC CCAGGCAGTG GGGCCTACCA ACGTCAGCCT CACCGTGACT
1981     AACATGCCAC CGGGCAAGCA CTTCCGGGTA GACGGCACCT CCGTGCTGAG AGGCTTCTCT
2041     TTCATGGAGC CAGTGCTGAT AGCAGTGCAA CCCCTCTTTG GCCCACGGGC AGGAGGCACC
2101     TGTCTCACTC TTGAAGGCCA GAGTCTGTCT GTAGGCACCA GCCGGGCTGT GCTGGTCAAT
2161     GGGACTGAGT GTCTGCTAGC ACGGGTCAGT GAGGGGCAGC TTTTATGTGC CACACCCCCT
2221     GGGGCCACGG TGGCCAGTGT CCCCCTTAGC CTGCAGGTGG GGGGTGCCCA GGTACCTGGT
2281     TCCTGGACCT TCCAGTACAG AGAAGACCCT GTCGTGCTAA GCATCAGCCC CAACTGTGGC
2341     TACATCAACT CCCACATCAC CATCTGTGGC CAGCATCTAA CTTCAGCATG GCACTTAGTG
2401     CTGTCATTCC ATGACGGGCT TAGGGCAGTG GAAAGCAGGT GTGAGAGGCA GCTTCCAGAG
2461     CAGCAGCTGT GCCGCCTTCC TGAATATGTG GTCCGAGACC CCCAGGGATG GGTGGCAGGG
2521     AATCTGAGTG CCCGAGGGGA TGGAGCTGCT GGCTTTACAC TGCCTGGCTT TCGCTTCCTA
2581     CCCCCACCCC ATCCACCCAG TGCCAACCTA GTTCCACTGA AGCCTGAGGA GCATGCCATT
2641     AAGTTTGAGC TTGGCCAGGA TGGTGCCCCA TTGCAGGTCT GCGTAGATGG TGAATGTCAT
2701     ATCCTGGGTA GAGTGGTGCG GCCAGGGCCA GATGGGGTCC CACAGAGCAC GCTCCTTGGT
2761     ATCCTGCTGC CTTTGCTGCT GCTTGTGGCT GCACTGGCGA CTGCACTGGT CTTCAGCTAC
2821     TGGTGGCGGA GGAAGCAGCT AGTTCTTCCT CCCAACCTGA ATGACCTGGC ATCCCTGGAC
2881     CAGACTGCTG GAGCCACACC CCTGCCTATT CTGTACTCGG GCTCTGACTA CAGAAGTGGC
2941     CTTGCACTCC CTGCCATTGA TGGTCTGGAT TCCACCACTT GTGTCCATGG AGCATCCTTC
3001     TCCGATAGTG AAGATGAATC CTGTGTGCCA CTGCTGCGGA AAGAGTCCAT CCAGCTAAGG
3061     GACCTGGACT CTGCGCTCTT GGCTGAGGTC AAGGATGTGC TGATTCCCCA TGAGCGGGTG
3121     GTCACCCACA GTGACCGAGT CATTGGCAAA GGCCACTTTG GAGTTGTCTA CCACGGAGAA
3181     TACATAGACC AGGCCCAGAA TCGAATCCAA TGTGCCATCA AGTCACTAAG TCGCATCACA
3241     GAGATGCAGC AGGTGGAGGC CTTCCTGCGA GAGGGGCTGC TCATGCGTGG CCTGAACCAC
3301     CCGAATGTGC TGGCTCTCAT TGGTATCATG TTGCCACCTG AGGGCCTGCC CCATGTGCTG
3361     CTGCCCTATA TGTGCCACGG TGACCTGCTC CAGTTCATCC GCTCACCTCA GCGGAACCCC
3421     ACCGTGAAGG ACCTCATCAG CTTTGGCCTG CAGGTAGCCC GCGGCATGGA GTACCTGGCA
3481     GAGCAGAAGT TTGTGCACAG GGACCTGGCT GCGCGGAACT GCATGCTGGA CGAGTCATTC
3541     ACAGTCAAGG TGGCTGACTT TGGTTTGGCC CGCGACATCC TGGACAGGGA GTACTATAGT
3601     GTTCAACAGC ATCGCCACGC TCGCCTACCT GTGAAGTGGA TGGCGCTGGA GAGCCTGCAG
3661     ACCTATAGAT TTACCACCAA GTCTGATGTG TGGTCATTTG GTGTGCTGCT GTGGGAACTG
3721     CTGACACGGG GTGCCCCACC ATACCGCCAC ATTGACCCTT TTGACCTTAC CCACTTCCTG
3781     GCCCAGGGTC GGCGCCTGCC CCAGCCTGAG TATTGCCCTG ATTCTCTGTA CCAAGTGATG
3841     CAGCAATGCT GGGAGGCAGA CCCAGCAGTG CGACCCACCT TCAGAGTACT AGTGGGGGAG
3901     GTGGAGCAGA TAGTGTCTGC ACTGCTTGGG GACCATTATG TGCAGCTGCC AGCAACCTAC
3961     ATGAACTTGG GCCCCAGCAC CTCGCATGAG ATGAATGTGC GTCCAGAACA GCCGCAGTTC
4021     TCACCCATGC CAGGGAATGT ACGCCGGCCC CGGCCACTCT CAGAGCCTCC TCGGCCCACT
4081     TGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).