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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) Matriptase/ST14 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) Matriptase/ST14 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10284-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_021978.3
cDNA Size: 2568
Synonym: HAI, MT-SP1, MTSP-1, MTSP1, PRSS14, SNC19, TADG-15
Source: Human.
cDNA Description: ORF Clone of Homo sapiens suppression of tumorigenicity 14 (colon carcinoma) DNA.
Vector: Vector pMD18-T Simple
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the 1215C/T mutation not causing amino acid variation.
10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGGGGAGCG ATCGGGCCCG CAAGGGCGGA GGGGGCCCGA AGGACTTCGG CGCGGGACTC 61 AAGTACAACT CCCGGCACGA GAAAGTGAAT GGCTTGGAGG AAGGCGTGGA GTTCCTGCCA 121 GTCAACAACG TCAAGAAGGT GGAAAAGCAT GGCCCGGGGC GCTGGGTGGT GCTGGCAGCC 181 GTGCTGATCG GCCTCCTCTT GGTCTTGCTG GGGATCGGCT TCCTGGTGTG GCATTTGCAG 241 TACCGGGACG TGCGTGTCCA GAAGGTCTTC AATGGCTACA TGAGGATCAC AAATGAGAAT 301 TTTGTGGATG CCTACGAGAA CTCCAACTCC ACTGAGTTTG TAAGCCTGGC CAGCAAGGTG 361 AAGGACGCGC TGAAGCTGCT GTACAGCGGA GTCCCATTCC TGGGCCCCTA CCACAAGGAG 421 TCGGCTGTGA CGGCCTTCAG CGAGGGCAGC GTCATCGCCT ACTACTGGTC TGAGTTCAGC 481 ATCCCGCAGC ACCTGGTGGA GGAGGCCGAG CGCGTCATGG CCGAGGAGCG CGTAGTCATG 541 CTGCCCCCGC GGGCGCGCTC CCTGAAGTCC TTTGTGGTCA CCTCAGTGGT GGCTTTCCCC 601 ACGGACTCCA AAACAGTACA GAGGACCCAG GACAACAGCT GCAGCTTTGG CCTGCACGCC 661 CGCGGTGTGG AGCTGATGCG CTTCACCACG CCCGGCTTCC CTGACAGCCC CTACCCCGCT 721 CATGCCCGCT GCCAGTGGGC CCTGCGGGGG GACGCCGACT CAGTGCTGAG CCTCACCTTC 781 CGCAGCTTTG ACCTTGCGTC CTGCGACGAG CGCGGCAGCG ACCTGGTGAC GGTGTACAAC 841 ACCCTGAGCC CCATGGAGCC CCACGCCCTG GTGCAGTTGT GTGGCACCTA CCCTCCCTCC 901 TACAACCTGA CCTTCCACTC CTCCCAGAAC GTCCTGCTCA TCACACTGAT AACCAACACT 961 GAGCGGCGGC ATCCCGGCTT TGAGGCCACC TTCTTCCAGC TGCCTAGGAT GAGCAGCTGT 1021 GGAGGCCGCT TACGTAAAGC CCAGGGGACA TTCAACAGCC CCTACTACCC AGGCCACTAC 1081 CCACCCAACA TTGACTGCAC ATGGAACATT GAGGTGCCCA ACAACCAGCA TGTGAAGGTG 1141 CGCTTCAAAT TCTTCTACCT GCTGGAGCCC GGCGTGCCTG CGGGCACCTG CCCCAAGGAC 1201 TACGTGGAGA TCAATGGGGA GAAATACTGC GGAGAGAGGT CCCAGTTCGT CGTCACCAGC 1261 AACAGCAACA AGATCACAGT TCGCTTCCAC TCAGATCAGT CCTACACCGA CACCGGCTTC 1321 TTAGCTGAAT ACCTCTCCTA CGACTCCAGT GACCCATGCC CGGGGCAGTT CACGTGCCGC 1381 ACGGGGCGGT GTATCCGGAA GGAGCTGCGC TGTGATGGCT GGGCCGACTG CACCGACCAC 1441 AGCGATGAGC TCAACTGCAG TTGCGACGCC GGCCACCAGT TCACGTGCAA GAACAAGTTC 1501 TGCAAGCCCC TCTTCTGGGT CTGCGACAGT GTGAACGACT GCGGAGACAA CAGCGACGAG 1561 CAGGGGTGCA GTTGTCCGGC CCAGACCTTC AGGTGTTCCA ATGGGAAGTG CCTCTCGAAA 1621 AGCCAGCAGT GCAATGGGAA GGACGACTGT GGGGACGGGT CCGACGAGGC CTCCTGCCCC 1681 AAGGTGAACG TCGTCACTTG TACCAAACAC ACCTACCGCT GCCTCAATGG GCTCTGCTTG 1741 AGCAAGGGCA ACCCTGAGTG TGACGGGAAG GAGGACTGTA GCGACGGCTC AGATGAGAAG 1801 GACTGCGACT GTGGGCTGCG GTCATTCACG AGACAGGCTC GTGTTGTTGG GGGCACGGAT 1861 GCGGATGAGG GCGAGTGGCC CTGGCAGGTA AGCCTGCATG CTCTGGGCCA GGGCCACATC 1921 TGCGGTGCTT CCCTCATCTC TCCCAACTGG CTGGTCTCTG CCGCACACTG CTACATCGAT 1981 GACAGAGGAT TCAGGTACTC AGACCCCACG CAGTGGACGG CCTTCCTGGG CTTGCACGAC 2041 CAGAGCCAGC GCAGCGCCCC TGGGGTGCAG GAGCGCAGGC TCAAGCGCAT CATCTCCCAC 2101 CCCTTCTTCA ATGACTTCAC CTTCGACTAT GACATCGCGC TGCTGGAGCT GGAGAAACCG 2161 GCAGAGTACA GCTCCATGGT GCGGCCCATC TGCCTGCCGG ACGCCTCCCA TGTCTTCCCT 2221 GCCGGCAAGG CCATCTGGGT CACGGGCTGG GGACACACCC AGTATGGAGG CACTGGCGCG 2281 CTGATCCTGC AAAAGGGTGA GATCCGCGTC ATCAACCAGA CCACCTGCGA GAACCTCCTG 2341 CCGCAGCAGA TCACGCCGCG CATGATGTGC GTGGGCTTCC TCAGCGGCGG CGTGGACTCC 2401 TGCCAGGGTG ATTCCGGGGG ACCCCTGTCC AGCGTGGAGG CGGATGGGCG GATCTTCCAG 2461 GCCGGTGTGG TGAGCTGGGG AGACGGCTGC GCTCAGAGGA ACAAGCCAGG CGTGTACACA 2521 AGGCTCCCTC TGTTTCGGGA CTGGATCAAA GAGAACACTG GGGTATAG
Quality Control: cDNA sequencing results is available upon request.
