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쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-Myc 태그

데이터시트리뷰관련제품프로토콜
Mouse AK4 cDNA 클론 제품 정보
Gene_bank_ref_id:NM_001177602.1
cDNA 크기:672bp
cDNA 설명:Full length Clone DNA of Mus musculus adenylate kinase 4 with N terminal Myc tag.
유전자 동의어:Ak3, AK 4, Ak-3, Ak-4, Ak3l1, D4Ertd274e
:Mouse
벡터:pCMV3-N-Myc
Plasmid:
제한 사이트:
태그 씨퀀스:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
염기서열 설명:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-Myc 태그 on other vectors
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그MG52601-ACG265260
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그MG52601-ACR265260
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그MG52601-ANG265260
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그MG52601-ANR265260
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-Flag 태그MG52601-CF229890
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-His 태그MG52601-CH229890
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-Myc 태그MG52601-CM229890
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-HA 태그MG52601-CY229890
Mouse AK4 Gene cDNA clone plasmidMG52601-G88420
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-Flag 태그MG52601-NF229890
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-His 태그MG52601-NH229890
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-Myc 태그MG52601-NM229890
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-HA 태그MG52601-NY229890
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone in cloning vectorMG52601-U88420
쥐 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmidMG52601-UT229890
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연구배경

Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

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Cat No: MG52601-NM
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재고정보2-3 weeks
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