Cofilin cDNA ORF Clone, Mouse, N-Myc tag General Information
Identical with the Gene Bank Ref. ID sequence.
Full length Clone DNA of Mouse cofilin 1, non-muscle with N terminal Myc tag.
Enhanced CMV promoter
KpnI + XbaI(6kb+0.55kb)
Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
Cofilin cDNA ORF Clone, Mouse, N-Myc tag Validated Images
Cofilin cDNA ORF Clone, Mouse, N-Myc tag Alternative Names
AA959946 cDNA ORF Clone, Mouse;Cof cDNA ORF Clone, Mouse;n-cofilin cDNA ORF Clone, Mouse
Cofilin Background Information
CFL1, also known as n-cofilin, is a member of the ADF/Cofilin family. This family comprises three genes: CFL1, CFL2 and DSTN (destrin). ADF/Cofilin family members bind G-actin monomers and depolymerize actin filaments through two mechanisms: severing and increasing the off-rate for actin monomers from the pointed end. Cofilin also binds with other proteins such as myosin, tropomyosin, α-actinin, gelsolin and scruin. These proteins compete with cofilin for actin binding. Сofilin also plays a role in innate immune response. CFL1 contains 1 ADF-H domain and is widely distributed in various tissues. It is important for normal progress through mitosis and normal cytokinesis.
cofilin 1 (non-muscle)
Lappalainen P. et al., 1997, Nature. 388 (6637): 78-82. Ichetovkin I. et al., 2000, Cell Motil. 45 (4): 293-306. Carlier MF. et al., 1997, J Cell Biol. 136 (6): 1307-22.