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쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone in cloning vector

데이터시트리뷰관련제품프로토콜
Mouse ENO1 cDNA 클론 제품 정보
Gene_bank_ref_id:NM_023119.2
cDNA 크기:1305bp
cDNA 설명:Full length Clone DNA of Mus musculus enolase 1, alpha non-neuron.
유전자 동의어:Eno-1, MBP-1, AL022784, 0610008I15
:Mouse
벡터:PGEM-T Vector
Plasmid:pGEM-mENO1
제한 사이트:
태그 씨퀀스:
염기서열 설명:Identical with the Gene Bank Ref. ID sequence except for the point mutation: 34G>A(E12K)
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone in cloning vector on other vectors
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그MG52810-ACG265260
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그MG52810-ACR265260
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그MG52810-ANG265260
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그MG52810-ANR265260
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-Flag 태그MG52810-CF229890
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-His 태그MG52810-CH229890
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-Myc 태그MG52810-CM229890
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-HA 태그MG52810-CY229890
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone in cloning vectorMG52810-G88420
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-Flag 태그MG52810-NF229890
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-His 태그MG52810-NH229890
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-Myc 태그MG52810-NM229890
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-HA 태그MG52810-NY229890
쥐 ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmidMG52810-UT229890
 발현 벡터에 대해 자세히 알아보기
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참고자료
  • Capello M, et al. (2011) a-Enolase: a promising therapeutic and diagnostic tumor target. FEBS J. 278(7): 1064-74.
  • Kang HJ, et al. (2008) Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme. Acta Crystallogr D Biol Crystallogr. 64(Pt 6): 651-7.
  • Lopez-Alemany R, et al. (2005) Alpha-enolase plasminogen receptor in myogenesis. Front Biosci. 10: 30-6.
  • Ejeskdr K, et al. (2005) Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cells induces cell death. BMC Cancer. 5: 161.
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    Cat No: MG52810-G
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