IL24 cDNA ORF Clone, Mouse, C-His tag

Cat: MG51067-CH

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IL24 cDNA ORF Clone, Mouse, C-His tag General Information

Gene

Species
Mouse
NCBI Ref Seq
RefSeq ORF Size
546 bp
Description
Full length Clone DNA of Mouse interleukin 24 with C terminal His tag.

Plasmid

Promoter
Enhanced CMV promoter
Vector
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression

Storage & Shipping

Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

IL24 cDNA ORF Clone, Mouse, C-His tag Alternative Names

FISP cDNA ORF Clone, Mouse;Mda-7 cDNA ORF Clone, Mouse;Mda7 cDNA ORF Clone, Mouse;St16 cDNA ORF Clone, Mouse

IL24 Background Information

Interleukin-24 (IL-24) also known as Melanoma differentiation-associated gene 7 protein (MDA-7) is a member of the IL10 family of cytokines. IL-24/MDA-7/IL24 can induce apoptosis selectively in various cancer cells. Overexpression of IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Human IL-24/MDA-7/IL24 is secreted by activated peripheral blood mononuclear cells and is the ligand for two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. Northern blot analysis revealed IL-24/MDA-7/IL24 expression in human tissues associated with the immune system such as spleen, thymus, peripheral blood leukocytes and normal melanocytes. IL-24/MDA-7/IL24 binding to either its endogenous receptors on human keratinocytes or to ectopically expressed receptors on baby hamster kidney cells leads to activation of the signal transducers and activators of transcription.

Full Name
interleukin 24
References
  • Wang M, et al.. (2002) Interleukin 24 (MDA-7/MOB-5) signals through two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. J Biol Chem. 277(9): 7341-7.
  • Sauane M, et al.. (2003) MDA-7/IL-24: novel cancer growth suppressing and apoptosis inducing cytokine. Cytokine Growth Factor Rev. 14(1): 35-51.
  • Sarkar D, et al.. (2002) mda-7 (IL-24) Mediates selective apoptosis in human melanoma cells by inducing the coordinated overexpression of the GADD family of genes by means of p38 MAPK. Proc Natl Acad Sci U S A. 99(15): 10054-9.
  • IL24 cDNA ORF Clone, Rat, Related Products

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    IL24 cDNA ORF Clone, Rat, N-HA tag RG80464-NY
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    IL24 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    IL24 cDNA ORF Clone in Cloning Vector, Mouse MG51067-G
    IL24 cDNA ORF Clone, Mouse, untagged MG51067-UT
    IL24 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51067-ACGLN
    IL24 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51067-ACG
    IL24 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51067-ACR
    IL24 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51067-CF
    IL24 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51067-NF
    IL24 cDNA ORF Clone, Mouse, N-His tag MG51067-NH
    IL24 cDNA ORF Clone, Mouse, C-HA tag MG51067-CY
    IL24 cDNA ORF Clone, Mouse, N-HA tag MG51067-NY
    IL24 cDNA ORF Clone, Mouse, C-Myc tag MG51067-CM
    IL24 cDNA ORF Clone, Mouse, N-Myc tag MG51067-NM
    IL24 qPCR Primer Pairs, Mouse MP201022

    IL24 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    IL24 cDNA ORF Clone in Cloning Vector, Human HG12265-G
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    IL24 cDNA ORF Clone, Human, C-GFPSpark® tag HG12265-ACG
    IL24 cDNA ORF Clone, Human, C-OFPSpark® tag HG12265-ACR
    IL24 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG12265-CF
    IL24 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG12265-NF
    IL24 cDNA ORF Clone, Human, C-His tag HG12265-CH
    IL24 cDNA ORF Clone, Human, N-His tag HG12265-NH
    IL24 cDNA ORF Clone, Human, C-HA tag HG12265-CY
    IL24 cDNA ORF Clone, Human, N-HA tag HG12265-NY
    IL24 cDNA ORF Clone, Human, C-Myc tag HG12265-CM
    IL24 cDNA ORF Clone, Human, N-Myc tag HG12265-NM
    IL24 qPCR Primer Pairs, Human HP101878
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