PDE4B cDNA ORF Clone, Mouse, N-His tag General Information
Full length Clone DNA of Mouse phosphodiesterase 4B, cAMP specific with N terminal His tag.
Enhanced CMV promoter
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
PDE4B cDNA ORF Clone, Mouse, N-His tag Alternative Names
Dpde4 cDNA ORF Clone, Mouse;dunce cDNA ORF Clone, Mouse;R74983 cDNA ORF Clone, Mouse
PDE4B Background Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4B, also known as PDE4B and DPDE4, is a member of the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. Cyclic nucleotide phosphodiesterases (PDEs) comprise a large family of enzymes that catalyze the hydrolysis of cAMP or cGMP and are implicated in various diseases. The crystal structures reveal a common scheme of inhibitor binding to the PDEs: (i) a hydrophobic clamp formed by highly conserved hydrophobic residues that sandwich the inhibitor in the active site; (ii) hydrogen bonding to an invariant glutamine that controls the orientation of inhibitor binding. A scaffold can be readily identified for any given inhibitor based on the formation of these two types of conserved interactions. These structural insights will enable the design of isoform-selective inhibitors with improved binding affinity and should facilitate the discovery of more potent and selective PDE inhibitors for the treatment of a variety of diseases. PDE4B / DPDE4 hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. It is expressed in brain, heart, lung and skeletal muscle. PDE4B / DPDE4 may be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents
phosphodiesterase 4B, cAMP-specific
Bolger G.et al., 1993, Mol. Cell. Biol. 13:6558-71. Card G.L.et al., 2004, Structure 12:2233-47. Card G.L.et al., 2005, Nat. Biotechnol. 23:201-7. Wang H.et al., 2007, Biochem. J. 408:193-201. Hamblin J.N. et al., 2008, Bioorg. Med. Chem. Lett. 18: 4237-41.