Src cDNA ORF Clone, Mouse, C-HA tag General Information
Full length Clone DNA of Mouse Rous sarcoma oncogene transcript variant 1 with C terminal HA tag.
Enhanced CMV promoter
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
Src cDNA ORF Clone, Mouse, C-HA tag Alternative Names
AW259666 cDNA ORF Clone, Mouse;pp60c-src cDNA ORF Clone, Mouse
Src Background Information
Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.
SRC proto-oncogene, non-receptor tyrosine kinase
Juan Ma. et al., 2003, Neuroscience Letters. 348 (3): 185-189. Czernilofsky AP. et al., 1980, Nature. 287: 198-203. Beischlag TV. et al., 2002, Molecular and cellular biology. 22 (12): 4319-33.