SRFBP1 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag

Cat: MG51646-CF
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SRFBP1 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag General Information
Gene
Species
Mouse
NCBI Ref Seq
RefSeq ORF Size
1326 bp
Description
Full length Clone DNA of Mouse serum response factor binding protein 1 with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

SRFBP1 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Alternative Names
2810036K01Rik cDNA ORF Clone, Mouse;p49/STRAP cDNA ORF Clone, Mouse
SRFBP1 Background Information

SRFBP1 contains 7 WD repeats and belongs to the WD repeat STRAP family. SRFBP1 may play a role in the cellular distribution of the SMN complex. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. SRFBP1 negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein. SRFBP1 may be involved in regulating transcriptional activation of cardiac genes during the aging process. It also may play a role in biosynthesis and/or processing of SLC2A4 in adipose cells.

Full Name
serum response factor binding protein 1
References
  • Datta PK, et al. (1999) Identification of STRAP, a novel WD domain protein in transforming growth factor-beta signaling. J Biol Chem. 273(52):34671-4.
  • Datta PK, et al. (1998) Identification of STRAP, a novel WD domain protein in transforming growth factor-beta signaling. J Biol Chem. 273(52):34671-4.
  • Datta PK, et al. (2000) STRAP and Smad7 synergize in the inhibition of transforming growth factor beta signaling. Mol Cell Biol. 20(9):3157-67.
  • SRFBP1 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    SRFBP1 cDNA ORF Clone in Cloning Vector, Mouse MG51646-G
    SRFBP1 cDNA ORF Clone, Mouse, untagged MG51646-UT
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    SRFBP1 cDNA ORF Clone, Mouse, C-His tag MG51646-CH
    SRFBP1 cDNA ORF Clone, Mouse, N-His tag MG51646-NH
    SRFBP1 cDNA ORF Clone, Mouse, C-HA tag MG51646-CY
    SRFBP1 cDNA ORF Clone, Mouse, N-HA tag MG51646-NY
    SRFBP1 cDNA ORF Clone, Mouse, C-Myc tag MG51646-CM
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    SRFBP1 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    SRFBP1 cDNA ORF Clone in Cloning Vector, Human HG14461-G
    SRFBP1 cDNA ORF Clone, Human, untagged HG14461-UT
    SRFBP1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG14461-ACGLN
    SRFBP1 cDNA ORF Clone, Human, C-GFPSpark® tag HG14461-ACG
    SRFBP1 cDNA ORF Clone, Human, N-GFPSpark® tag HG14461-ANG
    SRFBP1 cDNA ORF Clone, Human, C-OFPSpark® tag HG14461-ACR
    SRFBP1 cDNA ORF Clone, Human, N-OFPSpark® tag HG14461-ANR
    SRFBP1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG14461-CF
    SRFBP1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG14461-NF
    SRFBP1 cDNA ORF Clone, Human, C-His tag HG14461-CH
    SRFBP1 cDNA ORF Clone, Human, N-His tag HG14461-NH
    SRFBP1 cDNA ORF Clone, Human, C-HA tag HG14461-CY
    SRFBP1 cDNA ORF Clone, Human, N-HA tag HG14461-NY
    SRFBP1 cDNA ORF Clone, Human, C-Myc tag HG14461-CM
    SRFBP1 cDNA ORF Clone, Human, N-Myc tag HG14461-NM
    SRFBP1 qPCR Primer Pairs, Human HP103109
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