Smad5 cDNA ORF Clone, Mouse, N-His tag General Information
Full length Clone DNA of Mouse Mothers against decapentaplegic homolog 5 with N terminal His tag.
Enhanced CMV promoter
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
Smad5 cDNA ORF Clone, Mouse, N-His tag Alternative Names
1110051M15Rik cDNA ORF Clone, Mouse;AI451355 cDNA ORF Clone, Mouse;Dwf-C cDNA ORF Clone, Mouse;Madh5 cDNA ORF Clone, Mouse;MusMLP cDNA ORF Clone, Mouse
Smad5 Background Information
SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
SMAD family member 5
Vinayagam A. et al., 2011, Sci Signal. 4 (189): rs8. Sangadala S. et al., 2007, J Biomol Struct Dyn. 25 (1): 11-23. Riggins GJ. et al., 1996, Nat Genet. 13 (3): 347-9.