휴먼(Human) NLGN1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

NLGN1 cDNA Clone Datasheet

NLGN1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_014932.2
• cDNA Size: 2472
• Gene Synonym: KIAA1070; MGC45115; NLGN1
• Source: Human

NLGN1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens neuroligin 1 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NLGN1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NLGN1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

NLGN1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCACTGC CCAGATGCAC GTGGCCAAAT TATGTTTGGA GAGCAGTGAT GGCATGCTTG
61       GTACACCGGG GATTGGGTGC CCCATTGACT CTCTGTATGT TGGGATGTTT GCTTCAGGCT
121      GGCCATGTGC TATCACAAAA ATTGGATGAT GTGGACCCAC TGGTGGCTAC CAACTTTGGA
181      AAGATAAGAG GGATTAAGAA GGAACTCAAT AATGAAATTT TGGGGCCTGT TATTCAATTT
241      CTTGGGGTTC CATATGCAGC CCCACCAACA GGGGAACGTC GTTTTCAGCC TCCAGAACCA
301      CCATCTCCCT GGTCAGATAT CAGAAATGCC ACTCAATTTG CTCCTGTGTG TCCCCAGAAT
361      ATCATTGATG GCAGATTGCC AGAAGTCATG CTTCCTGTGT GGTTTACTAA TAACTTGGAT
421      GTGGTTTCAT CATATGTGCA AGACCAGAGC GAAGACTGCC TATATTTAAA TATATATGTC
481      CCGACTGAGG ATGATATTCG GGACAGTGGG GGTCCCAAAC CAGTGATGGT GTATATCCAT
541      GGTGGCTCAT ATATGGAAGG TACTGGAAAT TTATATGATG GAAGTGTCTT GGCAAGTTAT
601      GGCAATGTGA TCGTCATCAC AGTCAACTAT CGACTTGGAG TACTCGGTTT CTTGAGTACA
661      GGCGATCAGG CTGCAAAGGG GAACTATGGA CTCCTTGATC TCATACAAGC TTTAAGATGG
721      ACTAGTGAAA ACATTGGATT CTTTGGTGGT GACCCCTTAA GAATCACTGT TTTTGGATCT
781      GGTGCTGGGG GTTCATGTGT CAACCTGCTG ACTTTATCCC ATTATTCTGA AGGTAACCGT
841      TGGAGCAATT CAACCAAAGG ACTTTTTCAA CGAGCAATAG CTCAAAGTGG AACAGCCCTT
901      TCCAGCTGGG CTGTTAGTTT TCAACCTGCA AAATATGCTA GAATGTTGGC CACAAAAGTT
961      GGTTGCAATG TTTCAGATAC AGTAGAGTTA GTGGAATGCC TACAGAAGAA GCCTTACAAA
1021     GAACTTGTTG ACCAAGATAT TCAACCAGCT CGATACCACA TAGCCTTTGG ACCTGTGATT
1081     GATGGTGATG TAATACCAGA CGACCCCCAG ATATTGATGG AGCAAGGAGA GTTTCTCAAC
1141     TATGATATAA TGTTAGGAGT GAACCAAGGG GAAGGGTTAA AATTTGTTGA AAATATAGTA
1201     GATAGCGATG ATGGTATATC AGCTAGTGAT TTTGACTTTG CTGTTTCAAA TTTTGTTGAT
1261     AATTTATATG GATATCCTGA AGGCAAAGAT GTTTTGAGAG AAACCATTAA GTTCATGTAT
1321     ACTGACTGGG CTGACCGTCA TAACCCTGAA ACCAGAAGAA AGACATTACT GGCTTTGTTT
1381     ACGGACCATC AGTGGGTGGC ACCAGCTGTA GCCACAGCGG ATCTTCACTC AAACTTTGGT
1441     TCACCTACGT ACTTCTATGC CTTTTACCAT CATTGCCAAA CAGATCAGGT TCCAGCTTGG
1501     GCTGATGCAG CCCACGGAGA CGAGGTTCCC TATGTACTGG GAATCCCCAT GATTGGCCCT
1561     ACAGAGTTAT TTCCTTGCAA TTTCTCCAAA AATGATGTGA TGCTGAGTGC AGTTGTAATG
1621     ACATACTGGA CAAATTTTGC TAAAACTGGT GACCCAAATC AACCAGTCCC TCAAGACACG
1681     AAATTCATTC ATACCAAACC CAACCGTTTT GAAGAAGTAG CATGGACCAG ATATTCCCAG
1741     AAAGACCAAC TTTATCTCCA TATTGGATTA AAACCAAGAG TTAAAGAACA TTACAGAGCC
1801     AATAAGGTGA ACCTCTGGTT GGAGTTGGTA CCTCATCTGC ATAATCTCAA TGACATTTCT
1861     CAGTATACCT CTACAACAAC TAAAGTGCCA TCAACTGACA TCACTTTCAG ACCTACGAGA
1921     AAAAATTCTG TACCTGTCAC GTCAGCCTTT CCCACTGCCA AGCAGGATGA TCCCAAACAA
1981     CAACCAAGTC CATTTTCAGT GGATCAAAGG GACTACTCAA CAGAGCTGAG TGTCACTATT
2041     GCAGTTGGAG CATCACTGCT GTTTCTGAAC ATCTTGGCCT TTGCAGCCCT GTACTACAAA
2101     AAGGATAAGA GGAGACATGA TGTTCACAGG AGATGCAGCC CTCAGCGCAC TACTACCAAT
2161     GATCTAACCC ATGCACAAGA AGAGGAAATC ATGTCCCTCC AAATGAAGCA CACTGATTTG
2221     GATCATGAAT GTGAGTCCAT TCATCCACAT GAGGTGGTTC TTCGGACCGC CTGTCCCCCA
2281     GATTACACAC TAGCTATGAG GAGGTCACCT GATGATGTTC CCTTAATGAC ACCCAACACC
2341     ATTACAATGA TTCCCAACAC TATACCAGGG ATTCAGCCCT TACACACATT CAATACATTT
2401     ACTGGAGGAC AGAACAATAC TCTGCCCCAT CCCCATCCCC ACCCCCATTC ACATTCAACA
2461     ACCAGGGTAT AG

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).