휴먼(Human) neuregulin 1 (NRG1) transcript variant HRG-beta1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

NRG1 cDNA Clone Datasheet

NRG1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013956.3
• cDNA Size: 1938
• Gene Synonym: GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; NRG1
• Source: Human

NRG1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens neuregulin 1, isformb1 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NRG1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NRG1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

NRG1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCCGAGC GCAAAGAAGG CAGAGGCAAA GGGAAGGGCA AGAAGAAGGA GCGAGGCTCC
61       GGCAAGAAGC CGGAGTCCGC GGCGGGCAGC CAGAGCCCAG CCTTGCCTCC CCGATTGAAA
121      GAGATGAAAA GCCAGGAATC GGCTGCAGGT TCCAAACTAG TCCTTCGGTG TGAAACCAGT
181      TCTGAATACT CCTCTCTCAG ATTCAAGTGG TTCAAGAATG GGAATGAATT GAATCGAAAA
241      AACAAACCAC AAAATATCAA GATACAAAAA AAGCCAGGGA AGTCAGAACT TCGCATTAAC
301      AAAGCATCAC TGGCTGATTC TGGAGAGTAT ATGTGCAAAG TGATCAGCAA ATTAGGAAAT
361      GACAGTGCCT CTGCCAATAT CACCATCGTG GAATCAAACG AGATCATCAC TGGTATGCCA
421      GCCTCAACTG AAGGAGCATA TGTGTCTTCA GAGTCTCCCA TTAGAATATC AGTATCCACA
481      GAAGGAGCAA ATACTTCTTC ATCTACATCT ACATCCACCA CTGGGACAAG CCATCTTGTA
541      AAATGTGCGG AGAAGGAGAA AACTTTCTGT GTGAATGGAG GGGAGTGCTT CATGGTGAAA
601      GACCTTTCAA ACCCCTCGAG ATACTTGTGC AAGTGCCCAA ATGAGTTTAC TGGTGATCGC
661      TGCCAAAACT ACGTAATGGC CAGCTTCTAC AAGCATCTTG GGATTGAATT TATGGAGGCG
721      GAGGAGCTGT ACCAGAAGAG AGTGCTGACC ATAACCGGCA TCTGCATCGC CCTCCTTGTG
781      GTCGGCATCA TGTGTGTGGT GGCCTACTGC AAAACCAAGA AACAGCGGAA AAAGCTGCAT
841      GACCGTCTTC GGCAGAGCCT TCGGTCTGAA CGAAACAATA TGATGAACAT TGCCAATGGG
901      CCTCACCATC CTAACCCACC CCCCGAGAAT GTCCAGCTGG TGAATCAATA CGTATCTAAA
961      AACGTCATCT CCAGTGAGCA TATTGTTGAG AGAGAAGCAG AGACATCCTT TTCCACCAGT
1021     CACTATACTT CCACAGCCCA TCACTCCACT ACTGTCACCC AGACTCCTAG CCACAGCTGG
1081     AGCAACGGAC ACACTGAAAG CATCCTTTCC GAAAGCCACT CTGTAATCGT GATGTCATCC
1141     GTAGAAAACA GTAGGCACAG CAGCCCAACT GGGGGCCCAA GAGGACGTCT TAATGGCACA
1201     GGAGGCCCTC GTGAATGTAA CAGCTTCCTC AGGCATGCCA GAGAAACCCC TGATTCCTAC
1261     CGAGACTCTC CTCATAGTGA AAGGTATGTG TCAGCCATGA CCACCCCGGC TCGTATGTCA
1321     CCTGTAGATT TCCACACGCC AAGCTCCCCC AAATCGCCCC CTTCGGAAAT GTCTCCACCC
1381     GTGTCCAGCA TGACGGTGTC CATGCCTTCC ATGGCGGTCA GCCCCTTCAT GGAAGAAGAG
1441     AGACCTCTAC TTCTCGTGAC ACCACCAAGG CTGCGGGAGA AGAAGTTTGA CCATCACCCT
1501     CAGCAGTTCA GCTCCTTCCA CCACAACCCC GCGCATGACA GTAACAGCCT CCCTGCTAGC
1561     CCCTTGAGGA TAGTGGAGGA TGAGGAGTAT GAAACGACCC AAGAGTACGA GCCAGCCCAA
1621     GAGCCTGTTA AGAAACTCGC CAATAGCCGG CGGGCCAAAA GAACCAAGCC CAATGGCCAC
1681     ATTGCTAACA GATTGGAAGT GGACAGCAAC ACAAGCTCCC AGAGCAGTAA CTCAGAGAGT
1741     GAAACAGAAG ATGAAAGAGT AGGTGAAGAT ACGCCTTTCC TGGGCATACA GAACCCCCTG
1801     GCAGCCAGTC TTGAGGCAAC ACCTGCCTTC CGCCTGGCTG ACAGCAGGAC TAACCCAGCA
1861     GGCCGCTTCT CGACACAGGA AGAAATCCAG GCCAGGCTGT CTAGTGTAAT TGCTAACCAA
1921     GACCCTATTG CTGTATAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).