마우스(Mouse) RTN4R/NOGOR 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

RTN4R cDNA Clone Datasheet

RTN4R cDNA Clone Product Information

• Gene Bank Ref.ID: NM_022982.2
• cDNA Size: 1422
• Gene Synonym: NgR; NOGOR
• Source: Mouse

RTN4R cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus reticulon 4 receptor DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

RTN4R cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

RTN4R cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

RTN4R cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGAGGG CGTCCTCCGG AGGAAGCAGG CTGCTGGCAT GGGTGTTATG GCTACAGGCC
61       TGGAGGGTAG CAACACCATG CCCTGGTGCT TGTGTGTGCT ACAATGAGCC CAAGGTAACA
121      ACAAGCTGCC CCCAGCAGGG TCTGCAGGCT GTGCCCACTG GCATCCCAGC CTCTAGCCAG
181      CGAATCTTCC TGCATGGCAA CCGAATCTCT CACGTGCCAG CTGCGAGCTT CCAGTCATGC
241      CGAAATCTCA CTATCCTGTG GCTGCACTCT AATGCGCTGG CTCGGATCGA TGCTGCTGCC
301      TTCACTGGTC TGACCCTCCT GGAGCAACTA GATCTTAGTG ATAATGCACA GCTTCATGTC
361      GTGGACCCTA CCACGTTCCA CGGCCTGGGC CACCTGCACA CACTGCACCT AGACCGATGT
421      GGCCTGCGGG AGCTGGGTCC CGGCCTATTC CGTGGACTAG CAGCTCTGCA GTACCTCTAC
481      CTACAAGACA ACAATCTGCA GGCACTCCCT GACAACACCT TTCGAGACCT GGGCAACCTC
541      ACGCATCTCT TTCTGCATGG CAACCGTATC CCCAGTGTGC CTGAGCACGC TTTCCGTGGC
601      CTGCACAGTC TTGACCGCCT CCTCTTGCAC CAGAACCATG TGGCTCGTGT GCACCCACAT
661      GCCTTCCGGG ACCTTGGCCG CCTCATGACC CTCTACCTGT TTGCCAACAA CCTCTCCATG
721      CTGCCTGCAG AGGTCCTAAT GCCCCTGAGG TCTCTGCAGT ACCTGCGACT CAATGACAAC
781      CCCTGGGTGT GTGACTGCCG GGCACGTCCA CTCTGGGCCT GGCTGCAGAA GTTCCGAGGT
841      TCCTCATCAG AGGTGCCCTG CAACCTGCCC CAACGCCTGG CAGACCGTGA TCTTAAGCGC
901      CTCGCTGCCA GTGACCTAGA GGGCTGTGCT GTGGCTTCAG GACCCTTCCG TCCCATCCAG
961      ACCAGTCAGC TCACTGATGA GGAGCTGCTG AGCCTCCCCA AGTGCTGCCA GCCAGATGCT
1021     GCAGACAAAG CCTCAGTACT GGAACCCGGG AGGCCAGCTT CTGCCGGAAA CGCCCTCAAG
1081     GGACGTGTGC CTCCCGGTGA CACTCCACCA GGCAATGGCT CAGGCCCTCG GCACATCAAT
1141     GACTCTCCAT TTGGAACTTT GCCCAGCTCT GCAGAGCCCC CACTGACTGC CCTGCGGCCT
1201     GGGGGTTCCG AGCCACCAGG ACTTCCCACC ACTGGTCCCC GCAGGAGGCC AGGTTGTTCC
1261     CGGAAGAATC GCACCCGCAG CCACTGCCGT CTGGGCCAGG CGGGAAGTGG GGCCAGTGGA
1321     ACAGGGGACG CAGAGGGTTC AGGGGCTCTG CCTGCTCTGG CCTGCAGCCT TGCTCCTCTG
1381     GGCCTTGCAC TGGTACTTTG GACAGTGCTT GGGCCCTGCT GA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).