마우스(Mouse) NDP/EVR2 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

NDP cDNA Clone Datasheet

NDP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010883.2
• cDNA Size: 396
• Gene Synonym: ND; Ndph
• Source: Mouse

NDP cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Norrie disease (pseudoglioma) (human) DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NDP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NDP cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

NDP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGAAATC ATGTACTAGC TGCATCCATT TCTATGCTCT CCCTGCTGGC CATAATGGGA
61       GATACAGACA GCAAAACAGA CAGTTCATTT CTGATGGACT CTCAACGCTG CATGAGACAC
121      CATTATGTCG ATTCTATCAG TCACCCACTG TACAAATGTA GCTCAAAGAT GGTGCTCCTG
181      GCCAGATGTG AGGGGCACTG CAGCCAGGCA TCACGCTCTG AGCCCTTGGT GTCCTTCAGC
241      ACTGTCCTCA AGCAACCTTT CCGTTCCTCC TGTCACTGCT GCCGACCCCA GACTTCCAAG
301      CTGAAGGCTC TGCGTCTGCG CTGCTCAGGG GGCATGCGAC TTACTGCCAC TTACCGGTAC
361      ATCCTCTCCT GTCACTGTGA GGAATGCAGC TCCTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).