마우스(Mouse) OSM 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

OSM cDNA Clone Datasheet

OSM cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001013365.1
• cDNA Size: 792
• Gene Synonym: OSM
• Source: Mouse

OSM cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Oncostatin M DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

OSM cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

OSM cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

OSM cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGACAC GGCTTCTAAG AACACTGCTC AGTTTGACCC TCAGTCTCCT CATCCTGAGC
61       ATGGCACTGG CCAATCGTGG CTGCTCCAAC TCTTCCTCTC AGCTCCTCAG CCAGCTGCAG
121      AATCAGGCGA ACCTCACGGG GAACACAGAA TCACTCTTGG AGCCCTATAT CCGCCTCCAA
181      AACCTGAACA CACCTGACCT GAGAGCTGCC TGCACCCAGC ACTCTGTGGC CTTCCCCAGT
241      GAGGACACAC TCCGGCAACT GAGCAAGCCT CACTTCCTGA GCACTGTGTA CACCACACTG
301      GACAGAGTCT TGTACCAACT GGATGCTTTA AGACAGAAAT TTCTGAAGAC TCCGGCTTTT
361      CCAAAGCTGG ACAGTGCCCG GCACAATATC CTCGGCATAA GGAACAATGT TTTCTGCATG
421      GCCCGGCTGC TCAACCACTC CCTGGAGATA CCTGAGCCCA CACAGACAGA CTCTGGGGCC
481      TCACGGTCCA CTACAACACC AGATGTCTTT AATACCAAGA TAGGCAGCTG TGGCTTTCTC
541      TGGGGATACC ATCGCTTCAT GGGCTCAGTG GGGAGGGTCT TCAGGGAATG GGACGATGGC
601      TCCACACGCA GCCGGAGACA GAGCCCGCTC CGGGCCCGGC GCAAGGGAAC CCGCAGAATC
661      CGGGTCCGGC ACAAGGGAAC CCGCAGAATC CGGGTCCGGC GCAAGGGAAC CCGCAGAATC
721      TGGGTCCGGC GCAAGGGATC CCGCAAAATC AGACCTTCCA GGAGCACCCA GAGCCCGACG
781      ACCAGGGCCT AG

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).