마우스(Mouse) SPP1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

SPP1 cDNA Clone Datasheet

SPP1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009263.2
• cDNA Size: 885
• Gene Synonym: OP; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1; AA960535; AI790405; minopontin
• Source: Mouse

SPP1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus secreted phosphoprotein 1 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

SPP1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SPP1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

SPP1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGATTGG CAGTGATTTG CTTTTGCCTG TTTGGCATTG CCTCCTCCCT CCCGGTGAAA
61       GTGACTGATT CTGGCAGCTC AGAGGAGAAG CTTTACAGCC TGCACCCAGA TCCTATAGCC
121      ACATGGCTGG TGCCTGACCC ATCTCAGAAG CAGAATCTCC TTGCGCCACA GAATGCTGTG
181      TCCTCTGAAG AAAAGGATGA CTTTAAGCAA GAAACTCTTC CAAGCAATTC CAATGAAAGC
241      CATGACCACA TGGACGACGA TGATGACGAT GATGATGACG ATGGAGACCA TGCAGAGAGC
301      GAGGATTCTG TGGACTCGGA TGAATCTGAC GAATCTCACC ATTCGGATGA GTCTGATGAG
361      ACCGTCACTG CTAGTACACA AGCAGACACT TTCACTCCAA TCGTCCCTAC AGTCGATGTC
421      CCCAACGGCC GAGGTGATAG CTTGGCTTAT GGACTGAGGT CAAAGTCTAG GAGTTTCCAG
481      GTTTCTGATG AACAGTATCC TGATGCCACA GATGAGGACC TCACCTCTCA CATGAAGAGC
541      GGTGAGTCTA AGGAGTCCCT CGATGTCATC CCTGTTGCCC AGCTTCTGAG CATGCCCTCT
601      GATCAGGACA ACAACGGAAA GGGCAGCCAT GAGTCAAGTC AGCTGGATGA ACCAAGTCTG
661      GAAACACACA GACTTGAGCA TTCCAAAGAG AGCCAGGAGA GTGCCGATCA GTCGGATGTG
721      ATCGATAGTC AAGCAAGTTC CAAAGCCAGC CTGGAACATC AGAGCCACAA GTTTCACAGC
781      CACAAGGACA AGCTAGTCCT AGACCCTAAG AGTAAGGAAG ATGATAGGTA TCTGAAATTC
841      CGAATTTCTC ATGAATTAGA GAGTTCATCT TCTGAGGTCA ACTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).