휴먼(Human) Spp1 transcript variant 1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10352-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_001040058.1

cDNA Size:   945

Synonym:  OPN, BNSP, BSPI, ETA-1, MGC110940

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens secreted phosphoprotein 1, transcript variant 1 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGAGAATTG CAGTGATTTG CTTTTGCCTC CTAGGCATCA CCTGTGCCAT ACCAGTTAAA
61       CAGGCTGATT CTGGAAGTTC TGAGGAAAAG CAGCTTTACA ACAAATACCC AGATGCTGTG
121      GCCACATGGC TAAACCCTGA CCCATCTCAG AAGCAGAATC TCCTAGCCCC ACAGAATGCT
181      GTGTCCTCTG AAGAAACCAA TGACTTTAAA CAAGAGACCC TTCCAAGTAA GTCCAACGAA
241      AGCCATGACC ACATGGATGA TATGGATGAT GAAGATGATG ATGACCATGT GGACAGCCAG
301      GACTCCATTG ACTCGAACGA CTCTGATGAT GTAGATGACA CTGATGATTC TCACCAGTCT
361      GATGAGTCTC ACCATTCTGA TGAATCTGAT GAACTGGTCA CTGATTTTCC CACGGACCTG
421      CCAGCAACCG AAGTTTTCAC TCCAGTTGTC CCCACAGTAG ACACATATGA TGGCCGAGGT
481      GATAGTGTGG TTTATGGACT GAGGTCAAAA TCTAAGAAGT TTCGCAGACC TGACATCCAG
541      TACCCTGATG CTACAGACGA GGACATCACC TCACACATGG AAAGCGAGGA GTTGAATGGT
601      GCATACAAGG CCATCCCCGT TGCCCAGGAC CTGAACGCGC CTTCTGATTG GGACAGCCGT
661      GGGAAGGACA GTTATGAAAC GAGTCAGCTG GATGACCAGA GTGCTGAAAC CCACAGCCAC
721      AAGCAGTCCA GATTATATAA GCGGAAAGCC AATGATGAGA GCAATGAGCA TTCCGATGTG
781      ATTGATAGTC AGGAACTTTC CAAAGTCAGC CGTGAATTCC ACAGCCATGA ATTTCACAGC
841      CATGAAGATA TGCTGGTTGT AGACCCCAAA AGTAAGGAAG AAGATAAACA CCTGAAATTT
901      CGTATTTCTC ATGAATTAGA TAGTGCATCT TCTGAGGTCA ATTAA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) Spp1 transcript variant 1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)