PCSK1 / NEC1 단백질(Protein) (His Tag)

Prohormone Convertase 1 / Neuroendocrine Convertase 1 Protein

PCSK1 / NEC1 Protein Product Information

• Synonym: PCSK1, BMIQ12, NEC1, PC1, PC3, SPC3
• Protein Construction: A DNA sequence encoding the human PCSK1 ( NP_000430.3 ) ( Met 1 - Arg 617 ) was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

PCSK1 / NEC1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 111
• Molecular Mass: The purified recombinant human PCSK1 consists of 518 amino acids after removal of the pro peptide due to autocatalytic processing, corresponding to the active form (aa 111-617), and has a predicted molecular mass of 57.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the active rh PCSK1 is approximately 66 kDa due to glycosylation.
• Formulation: Lyophilized from sterile 25mMTris, 150mM NaCl, pH 7.5
• SDS-PAGE: PCSK1 protein

PCSK1 / NEC1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PCSK1 / NEC1 Protein Description

Neuroendocrine convertase 1, also known as Prohormone convertase 1, Proprotein convertase 1, PCSK1 and NEC1, is an enzyme which belongs to the peptidase S8 family and Furin subfamily. PCSK1 is an enzyme that performs the proteolytic cleavage of prohormones to their intermediate (or sometimes completely cleaved) forms. It is present only in neuroendocrine cells such as brain, pituitary and adrenal, and most often cleaves after a pair of basic residues within prohormones but can occasionally cleave after a single arginine. It binds to a protein known as proSAAS, which also represents its endogenous inhibitor. PCSK1 is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK1 substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency). PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction. It is due to impaired processing of prohormones.

References

1. Jackson RS. et al., 2003, J. Clin. Invest. 112:1550-60.
2. Farooqi I.S. et al., 2007, J. Clin. Endocrinol. Metab. 92: 3369-73.
3. Benzinou,M. et al., 2008,Nat Genet. 40 (8):943-5.
4. Benzinou M. et al., 2008, Nat. Genet. 40:943-5.
5. Renström F, et al., 2009, Hum. Mol. Genet. 18 (8): 1489-96.