휴먼(Human) PD-ECGF 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10432-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_001953.3

cDNA Size:   1449

Synonym:  TP; ECGF1; MNGIE; PDECGF; hPD-ECGF; TYMP

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens thymidine phosphorylase DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the point mutation 972 C/T not causing the amino acid variation.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG
61       GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
121      CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
181      GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
241      CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
301      CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
361      GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
421      CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
481      ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
541      GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
601      GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
661      AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
721      GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
781      AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
841      GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
901      GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
961      GGGACTCAGG CTCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
1021     GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
1081     TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
1141     CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG
1201     CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC
1261     GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG
1321     CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA
1381     CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG
1441     CAGCAATAA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) PD-ECGF 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)