마우스(Mouse) PD1/PDCD1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

PDCD1 cDNA Clone Datasheet

PDCD1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008798.1
• cDNA Size: 867
• Gene Synonym: PD-1; Pdc1; Ly101
• Source: Mouse

PDCD1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus programmed cell death 1 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PDCD1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PDCD1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

PDCD1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGGGTCC GGCAGGTACC CTGGTCATTC ACTTGGGCTG TGCTGCAGTT GAGCTGGCAA
61       TCAGGGTGGC TTCTAGAGGT CCCCAATGGG CCCTGGAGGT CCCTCACCTT CTACCCAGCC
121      TGGCTCACAG TGTCAGAGGG AGCAAATGCC ACCTTCACCT GCAGCTTGTC CAACTGGTCG
181      GAGGATCTTA TGCTGAACTG GAACCGCCTG AGTCCCAGCA ACCAGACTGA AAAACAGGCC
241      GCCTTCTGTA ATGGTTTGAG CCAACCCGTC CAGGATGCCC GCTTCCAGAT CATACAGCTG
301      CCCAACAGGC ATGACTTCCA CATGAACATC CTTGACACAC GGCGCAATGA CAGTGGCATC
361      TACCTCTGTG GGGCCATCTC CCTGCACCCC AAGGCAAAAA TCGAGGAGAG CCCTGGAGCA
421      GAGCTCGTGG TAACAGAGAG AATCCTGGAG ACCTCAACAA GATATCCCAG CCCCTCGCCC
481      AAACCAGAAG GCCGGTTTCA AGGCATGGTC ATTGGTATCA TGAGTGCCCT AGTGGGTATC
541      CCTGTATTGC TGCTGCTGGC CTGGGCCCTA GCTGTCTTCT GCTCAACAAG TATGTCAGAG
601      GCCAGAGGAG CTGGAAGCAA GGACGACACT CTGAAGGAGG AGCCTTCAGC AGCACCTGTC
661      CCTAGTGTGG CCTATGAGGA GCTGGACTTC CAGGGACGAG AGAAGACACC AGAGCTCCCT
721      ACCGCCTGTG TGCACACAGA ATATGCCACC ATTGTCTTCA CTGAAGGGCT GGGTGCCTCG
781      GCCATGGGAC GTAGGGGCTC AGCTGATGGC CTGCAGGGTC CTCGGCCTCC AAGACATGAG
841      GATGGACATT GTTCTTGGCC TCTTTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).