휴먼(Human) PD1/PDCD1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10377-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_005018.2

cDNA Size:   867

Synonym:  PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens programmed cell death 1 (PDCD1) DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGCAGATCC CACAGGCGCC CTGGCCAGTC GTCTGGGCGG TGCTACAACT GGGCTGGCGG
61       CCAGGATGGT TCTTAGACTC CCCAGACAGG CCCTGGAACC CCCCCACCTT CTCCCCAGCC
121      CTGCTCGTGG TGACCGAAGG GGACAACGCC ACCTTCACCT GCAGCTTCTC CAACACATCG
181      GAGAGCTTCG TGCTAAACTG GTACCGCATG AGCCCCAGCA ACCAGACGGA CAAGCTGGCC
241      GCCTTCCCCG AGGACCGCAG CCAGCCCGGC CAGGACTGCC GCTTCCGTGT CACACAACTG
301      CCCAACGGGC GTGACTTCCA CATGAGCGTG GTCAGGGCCC GGCGCAATGA CAGCGGCACC
361      TACCTCTGTG GGGCCATCTC CCTGGCCCCC AAGGCGCAGA TCAAAGAGAG CCTGCGGGCA
421      GAGCTCAGGG TGACAGAGAG AAGGGCAGAA GTGCCCACAG CCCACCCCAG CCCCTCACCC
481      AGGCCAGCCG GCCAGTTCCA AACCCTGGTG GTTGGTGTCG TGGGCGGCCT GCTGGGCAGC
541      CTGGTGCTGC TAGTCTGGGT CCTGGCCGTC ATCTGCTCCC GGGCCGCACG AGGGACAATA
601      GGAGCCAGGC GCACCGGCCA GCCCCTGAAG GAGGACCCCT CAGCCGTGCC TGTGTTCTCT
661      GTGGACTATG GGGAGCTGGA TTTCCAGTGG CGAGAGAAGA CCCCGGAGCC CCCCGTGCCC
721      TGTGTCCCTG AGCAGACGGA GTATGCCACC ATTGTCTTTC CTAGCGGAAT GGGCACCTCA
781      TCCCCCGCCC GCAGGGGCTC AGCTGACGGC CCTCGGAGTG CCCAGCCACT GAGGCCTGAG
841      GATGGACACT GCTCTTGGCC CCTCTGA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) PD1/PDCD1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)