마우스(Mouse) PGF/PLGF 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

PGF cDNA Clone Datasheet

PGF cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008827.2
• cDNA Size: 477
• Gene Synonym: PIGF; Plgf; AI854365; Pgf
• Source: Mouse

PGF cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus placental growth factor DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PGF cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PGF cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

PGF cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGGTCA TGAAGCTGTT CACTTGCTTC TTACAGGTCC TAGCTGGGTT GGCTGTGCAT
61       TCCCAGGGGG CCCTGTCTGC TGGGAACAAC TCAACAGAAG TGGAAGTGGT GCCTTTCAAC
121      GAAGTGTGGG GTCGCAGCTA CTGTCGGCCC ATGGAGAAGC TGGTGTACAT CTTGGATGAA
181      TACCCTGATG AGGTGTCTCA CATATTCAGT CCGTCCTGTG TCCTTCTGAG TCGCTGTAGT
241      GGCTGCTGTG GTGATGAAGG TCTGCACTGT GTGCCGATAA AGACAGCCAA CATCACTATG
301      CAGATCTTGA AGATTCCCCC CAATCGGGAT CCACATTTCT ATGTGGAGAT GACATTTTCT
361      CAGGATGTGC TCTGTGAATG CAGACCTATT CTGGAGACGA CAAAGGCAGA AAGGAGGAAA
421      ACCAAGGGGA AGAGGAAGAG GAGTAGAAAC TCACAGACTG AGGAACCCCA CCCGTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).