휴먼(Human) PNCK 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

PNCK cDNA Clone Datasheet

PNCK cDNA Clone Product Information

• Gene Bank Ref.ID: BC064422.1
• cDNA Size: 726
• Gene Synonym: BSTK3; CaMK1b; FLJ50403; FLJ50549; FLJ56451; FLJ59811; MGC45419; PNCK
• Source: Human

PNCK cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens pregnancy up-regulated non-ubiquitously expressed CaM kinase DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PNCK cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PNCK cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

PNCK cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCGCG GCTCCTACAC AGAGAAGGAT GCCAGCCATC TGGTGGGTCA GGTCCTTGGC
61       GCCGTCTCCT ACCTGCACAG CCTGGGGATC GTGCACCGGG ACCTCAAGCC CGAAAACCTC
121      CTGTATGCCA CGCCCTTTGA GGACTCGAAG ATCATGGTCT CTGACTTTGG ACTCTCCAAA
181      ATCCAGGCTG GGAACATGCT AGGCACCGCC TGTGGGACCC CTGGATATGT GGCCCCAGAG
241      CTCTTGGAGC AGAAACCCTA CGGGAAGGCC GTAGATGTGT GGGCCCTGGG CGTCATCTCC
301      TACATCCTGC TGTGTGGGTA CCCCCCCTTC TACGACGAGA GCGACCCTGA GCTCTTCAGC
361      CAGATCCTGA GGGCCAGCTA TGAGTTTGAC TCTCCTTTCT GGGATGACAT CTCAGAATCA
421      GCCAAAGACT TCATCCGGCA CCTTCTGGAG CGAGACCCCC AGAAGAGGTT CACCTGCCAA
481      CAGGCCTTGC GGCACCTTTG GATCTCTGGG GACACAGCCT TCGACAGGGA CATCTTAGGC
541      TCTGTCAGTG AGCAGATCCG GAAGAACTTT GCTCGGACAC ACTGGAAGCG AGCCTTCAAT
601      GCCACCTCGT TCCTGCGCCA CATCCGGAAG CTGGGGCAGA TCCCAGAGGG CGAGGGGGCC
661      TCTGAGCAGG GCATGGCCCG CCACAGCCAC TCAGGCCTCC GTGCTGGCCA GCCCCCCAAG
721      TGGTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).