휴먼(Human) POT1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

POT1 cDNA Clone Datasheet

POT1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_015450.2
• cDNA Size: 1905
• Gene Synonym: HPOT1; DKFZp586D211; POT1
• Source: Human

POT1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens protection of telomeres 1 homolog (S. pombe) DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

POT1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

POT1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

POT1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCTTTGG TTCCAGCAAC AAATTATATA TATACACCCC TGAATCAACT TAAGGGTGGT
61       ACAATTGTCA ATGTCTATGG TGTTGTGAAG TTCTTTAAGC CCCCATATCT AAGCAAAGGA
121      ACTGATTATT GCTCAGTTGT AACTATTGTG GACCAGACAA ATGTAAAACT AACTTGCCTG
181      CTCTTTAGTG GAAACTATGA AGCCCTTCCA ATAATTTATA AAAATGGAGA TATTGTTCGC
241      TTTCACAGGC TGAAGATTCA AGTATATAAA AAGGAGACTC AGGGTATCAC CAGCTCTGGC
301      TTTGCATCTT TGACGTTTGA GGGAACTTTG GGAGCCCCTA TCATACCTCG CACTTCAAGC
361      AAGTATTTTA ACTTCACTAC TGAGGACCAC AAAATGGTAG AAGCCTTACG TGTTTGGGCA
421      TCTACTCATA TGTCACCGTC TTGGACATTA CTAAAATTGT GTGATGTTCA GCCAATGCAG
481      TATTTTGACC TGACTTGTCA GCTCTTGGGC AAAGCAGAAG TGGACGGAGC ATCATTTCTT
541      CTAAAGGTAT GGGATGGCAC CAGGACACCA TTTCCATCTT GGAGAGTCTT AATACAAGAC
601      CTTGTTCTTG AAGGTGATTT AAGTCACATC CATCGGCTAC AAAATCTGAC AATAGACATT
661      TTAGTCTACG ATAACCATGT TCATGTGGCA AGATCTCTGA AGGTTGGAAG CTTTCTTAGA
721      ATCTATAGCC TTCATACCAA ACTTCAATCA ATGAATTCAG AGAATCAGAC AATGTTAAGT
781      TTAGAGTTTC ATCTTCATGG AGGTACCAGT TACGGTCGGG GAATCAGGGT CTTGCCAGAA
841      AGTAACTCTG ATGTGGATCA ACTGAAAAAG GATTTAGAAT CTGCAAATTT GACAGCCAAT
901      CAGCATTCAG ATGTTATCTG TCAATCAGAA CCTGACGACA GCTTTCCAAG CTCTGGATCA
961      GTATCATTAT ACGAGGTAGA AAGATGTCAA CAGCTATCTG CTACAATACT TACAGATCAT
1021     CAGTATTTGG AGAGGACACC ACTATGTGCC ATTTTGAAAC AAAAAGCTCC TCAACAATAC
1081     CGCATCCGAG CAAAATTGAG GTCATATAAG CCCAGAAGAC TATTTCAGTC TGTTAAACTT
1141     CATTGCCCTA AATGTCATTT GCTGCAAGAA GTTCCACATG AGGGCGATTT GGATATAATT
1201     TTTCAGGATG GTGCAACTAA AACCCCAGAT GTCAAGCTAC AAAATACATC ATTATATGAT
1261     TCAAAAATCT GGACCACTAA AAATCAAAAA GGACGAAAAG TAGCAGTTCA TTTTGTGAAA
1321     AATAATGGTA TTCTCCCGCT TTCAAATGAA TGTCTACTTT TGATAGAAGG AGGTACACTC
1381     AGTGAAATTT GCAAACTCTC GAACAAGTTT AATAGTGTAA TTCCTGTGAG ATCTGGCCAC
1441     GAAGACCTGG AACTTTTGGA CCTTTCAGCA CCATTTCTTA TACAAGGAAC AATACATCAC
1501     TATGGATGTA AACAGTGTTC TAGTTTGAGA TCCATACAAA ATCTAAATTC CCTGGTTGAT
1561     AAAACATCGT GGATTCCTTC TTCTGTGGCA GAAGCACTGG GTATTGTACC CCTCCAATAT
1621     GTGTTTGTTA TGACCTTTAC ACTTGATGAT GGAACAGGAG TACTAGAAGC CTATCTCATG
1681     GATTCTGACA AATTCTTCCA GATTCCAGCA TCAGAAGTTC TGATGGATGA TGACCTTCAG
1741     AAAAGTGTGG ATATGATCAT GGATATGTTT TGTCCTCCAG GAATAAAAAT TGATGCATAT
1801     CCGTGGTTGG AATGCTTCAT CAAGTCATAC AATGTCACAA ATGGAACAGA TAATCAAATT
1861     TGCTATCAGA TTTTTGACAC CACAGTTGCA GAAGATGTAA TCTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).