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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) PRL 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) PRL 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10275-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_000948.3
cDNA Size: 684
Synonym: PRL
Source: Human.
cDNA Description: ORF Clone of Homo sapiens prolactin DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 46 C/G resulting in the amino acid Leu substitution by Val.
10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
1 ATGAACATCA AAGGATCGCC ATGGAAAGGG TCCCTCCTGC TGCTGGTGGT GTCAAACCTG 61 CTCCTGTGCC AGAGCGTGGC CCCCTTGCCC ATCTGTCCCG GCGGGGCTGC CCGATGCCAG 121 GTGACCCTTC GAGACCTGTT TGACCGCGCC GTCGTCCTGT CCCACTACAT CCATAACCTC 181 TCCTCAGAAA TGTTCAGCGA ATTCGATAAA CGGTATACCC ATGGCCGGGG GTTCATTACC 241 AAGGCCATCA ACAGCTGCCA CACTTCTTCC CTTGCCACCC CCGAAGACAA GGAGCAAGCC 301 CAACAGATGA ATCAAAAAGA CTTTCTGAGC CTGATAGTCA GCATATTGCG ATCCTGGAAT 361 GAGCCTCTGT ATCATCTGGT CACGGAAGTA CGTGGTATGC AAGAAGCCCC GGAGGCTATC 421 CTATCCAAAG CTGTAGAGAT TGAGGAGCAA ACCAAACGGC TTCTAGAGGG CATGGAGCTG 481 ATAGTCAGCC AGGTTCATCC TGAAACCAAA GAAAATGAGA TCTACCCTGT CTGGTCGGGA 541 CTTCCATCCC TGCAGATGGC TGATGAAGAG TCTCGCCTTT CTGCTTATTA TAACCTGCTC 601 CACTGCCTAC GCAGGGATTC ACATAAAATC GACAATTATC TCAAGCTCCT GAAGTGCCGA 661 ATCATCCACA ACAACAACTG CTAA
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
