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휴먼(Human) Pin1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)PDF Download

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HG10282-M
  YES       
Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_006221.2

cDNA Size:    492

Synonym:   PIN1, DOD, UBL5

Source:  Human. cDNA Description:  ORF Clone of Homo sapiens peptidylprolyl cis/trans isomerase, NIMA-interacting 1 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:   Identical with the Gene Bank Ref. ID sequence. 10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.
  1. Remove the marked edge in the center of the spot .
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to a heating block at 95oC for 15–30 min .
  4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGGCGGACG AGGAGAAGCT GCCGCCCGGC TGGGAGAAGC GCATGAGCCG CAGCTCAGGC
61       CGAGTGTACT ACTTCAACCA CATCACTAAC GCCAGCCAGT GGGAGCGGCC CAGCGGCAAC
121      AGCAGCAGTG GTGGCAAAAA CGGGCAGGGG GAGCCTGCCA GGGTCCGCTG CTCGCACCTG
181      CTGGTGAAGC ACAGCCAGTC ACGGCGGCCC TCGTCCTGGC GGCAGGAGAA GATCACCCGG
241      ACCAAGGAGG AGGCCCTGGA GCTGATCAAC GGCTACATCC AGAAGATCAA GTCGGGAGAG
301      GAGGACTTTG AGTCTCTGGC CTCACAGTTC AGCGACTGCA GCTCAGCCAA GGCCAGGGGA
361      GACCTGGGTG CCTTCAGCAG AGGTCAGATG CAGAAGCCAT TTGAAGACGC CTCGTTTGCG
421      CTGCGGACGG GGGAGATGAG CGGGCCCGTG TTCACGGATT CCGGCATCCA CATCATCCTC
481      CGCACTGAGT GA

Quality Control:   cDNA sequencing results is available upon request.


Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).