휴먼(Human) Podocan 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10303-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_153703.3

cDNA Size:    1986

Synonym:  PCAN, SLRR5A, MGC24995

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens podocan DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for two point mutations: 1559 T/C causing the 520Val to be replaced by Ala; and 1401G/A not causing any amino acid variation.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGAAGGAG CCCGAGCCCG CGGAGCGCAG CTGAGACTGG GGGAGCGCGT TCGGCCTGTG
61       GGGCGCCGCT CGGCGCCGGG GCGCAGCAGG TTCCGTCAGC CCTGGCGCCC AGGCGCATCT
121      GACTCGGCAC CCCCTGCAGG CACCATGGCC CAGAGCCGGG TGCTGCTGCT CCTGCTGCTG
181      CTGCCGCCAC AGCTGCACCT GGGACCTGTG CTTGCCGTGA GGGCCCCAGG ATTTGGCCGA
241      AGTGGCGGCC ACAGCCTGAG CCCCGAAGAG AACGAATTTG CGGAGGAGGA GCCGGTGCTG
301      GTACTGAGCC CTGAGGAGCC CGGGCCTGGC CCAGCCGCGG TCAGCTGCCC CCGAGACTGT
361      GCCTGTTCCC AGGAGGGCGT CGTGGACTGT GGCGGTATTG ACCTGCGTGA GTTCCCGGGG
421      GACCTGCCTG AGCACACCAA CCACCTATCT CTGCAGAACA ACCAGCTGGA AAAGATCTAC
481      CCTGAGGAGC TCTCCCGGCT GCACCGGCTG GAGACGCTGA ACCTGCAAAA CAACCGCCTG
541      ACTTCCCGAG GGCTCCCAGA GAAGGCGTTT GAGCATCTGA CCAACCTCAA TTACCTGTAC
601      TTGGCCAATA ACAAGCTGAC CTTGGCACCC CGCTTCCTGC CAAACGCCCT GATCAGTGTG
661      GACTTTGCTG CCAACTATCT CACCAAGATC TATGGGCTCA CCTTTGGCCA GAAGCCAAAC
721      TTGAGGTCTG TGTACCTGCA CAACAACAAG CTGGCAGACG CCGGGCTGCC GGACAACATG
781      TTCAACGGCT CCAGCAACGT CGAGGTCCTC ATCCTGTCCA GCAACTTCCT GCGCCACGTG
841      CCCAAGCACC TGCCGCCTGC CCTGTACAAG CTGCACCTCA AGAACAACAA GCTGGAGAAG
901      ATCCCCCCGG GGGCCTTCAG CGAGCTGAGC AGCCTGCGCG AGCTATACCT GCAGAACAAC
961      TACCTGACTG ACGAGGGCCT GGACAACGAG ACCTTCTGGA AGCTCTCCAG CCTGGAGTAC
1021     CTGGATCTGT CCAGCAACAA CCTGTCTCGG GTCCCAGCTG GGCTGCCGCG CAGCCTGGTG
1081     CTGCTGCACT TGGAGAAGAA CGCCATCCGG AGCGTGGACG CGAATGTGCT GACCCCCATC
1141     CGCAGCCTGG AGTACCTGCT GCTGCACAGC AACCAGCTGC GGGAGCAGGG CATCCACCCA
1201     CTGGCCTTCC AGGGCCTCAA GCGGTTGCAC ACGGTGCACC TGTACAACAA CGCGCTGGAG
1261     CGCGTGCCCA GTGGCCTGCC TCGCCGCGTG CGCACCCTCA TGATCCTGCA CAACCAGATC
1321     ACAGGCATTG GCCGCGAAGA CTTTGCCACC ACCTACTTCC TGGAGGAGCT CAACCTCAGC
1381     TACAACCGCA TCACCAGCCC ACAGGTGCAC CGCGACGCCT TCCGCAAGCT GCGCCTGCTG
1441     CGCTCGCTGG ACCTGTCGGG CAACCGGCTG CACACGCTGC CACCTGGGCT GCCTCGAAAT
1501     GTCCATGTGC TGAAGGTCAA GCGCAATGAG CTGGCTGCCT TGGCACGAGG GGCGCTGGCG
1561     GGCATGGCTC AGCTGCGTGA GCTGTACCTC ACCAGCAACC GACTGCGCAG CCGAGCCCTG
1621     GGCCCCCGTG CCTGGGTGGA CCTCGCCCAT CTGCAGCTGC TGGACATCGC CGGGAATCAG
1681     CTCACAGAGA TCCCCGAGGG GCTCCCCGAG TCACTTGAGT ACCTGTACCT GCAGAACAAC
1741     AAGATTAGTG CGGTGCCCGC CAATGCCTTC GACTCCACGC CCAACCTCAA GGGGATCTTT
1801     CTCAGGTTTA ACAAGCTGGC TGTGGGCTCC GTGGTGGACA GTGCCTTCCG GAGGCTGAAG
1861     CACCTGCAGG TCTTGGACAT TGAAGGCAAC TTAGAGTTTG GTGACATTTC CAAGGACCGT
1921     GGCCGCTTGG GGAAGGAAAA GGAGGAGGAG GAAGAGGAGG AGGAGGAGGA AGAGGAAACA
1981     AGATAG

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) Podocan 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)