마우스(Mouse) Prostasin/Prss8 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

PRSS8 cDNA Clone Datasheet

PRSS8 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_133351.2
• cDNA Size: 1020
• Gene Synonym: CAP1; mCAP1; C79772; AI313909; 2410039E18Rik
• Source: Mouse

PRSS8 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus protease, serine, 8 (prostasin) DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PRSS8 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRSS8 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

PRSS8 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCCTAA GGGTGGGCCT GGGACTTGGG CAGCTGGAAG CTGTGACCAT TCTGCTCCTT
61       CTCGGATTGC TCCAGTCGGG AATCCGAGCT GACGGGACTG AAGCCTCCTG TGGTGCCGTC
121      ATCCAGCCAC GCATCACCGG TGGTGGCAGT GCAAAGCCCG GTCAGTGGCC CTGGCAGGTC
181      AGCATCACCT ACGATGGCAA CCATGTTTGT GGCGGGTCGC TCGTGTCAAA TAAATGGGTG
241      GTGTCTGCTG CTCACTGCTT CCCCAGAGAA CACAGCAGGG AAGCGTATGA GGTGAAGCTG
301      GGGGCCCACC AGCTAGACTC CTACAGCAAT GACACTGTGG TCCACACAGT GGCTCAGATC
361      ATCACCCACT CAAGCTACCG AGAAGAGGGC TCCCAGGGGG ACATCGCGCT CATCCGCCTC
421      AGCAGTCCTG TCACCTTCTC CCGCTACATC AGACCCATCT GCCTCCCTGC AGCCAATGCC
481      TCCTTTCCCA ACGGCCTTCA CTGTACTGTC ACGGGATGGG GTCATGTGGC TCCTTCAGTG
541      AGCCTCCAGA CCCCTAGGCC TCTGCAGCAG CTCGAGGTAC CACTCATCAG CCGGGAAACC
601      TGTAGCTGCC TGTACAACAT TAATGCGGTG CCTGAAGAAC CGCACACTAT CCAGCAGGAC
661      ATGCTGTGTG CTGGCTATGT GAAGGGAGGC AAGGATGCCT GCCAGGGTGA CTCTGGGGGC
721      CCACTCTCTT GTCCCATGGA GGGCATCTGG TACTTGGCAG GCATTGTGAG TTGGGGTGAT
781      GCCTGTGGTG CCCCCAACAG GCCTGGAGTA TACACTCTGA CTTCTACCTA TGCTTCCTGG
841      ATCCACCACC ATGTGGCAGA GCTCCAGCCT CGAGTTGTCC CCCAGACTCA GGAGTCCCAG
901      CCGGATGGCC ACCTCTGCAA CCATCATCCT GTCTTCAGCT CAGCGGCAGC CCCGAAATTG
961      TTAAGGCCCG TACTTTTCCT GCCACTTGGT CTGACCCTGG GCCTCTTGTC TTTGGCTTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).