휴먼(Human) RCAN3/DSCR1L2 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10294-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_013441.2

cDNA Size:    726

Synonym:  RCN3; MCIP3; hRCN3; DSCR1L2

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens RCAN family member 3 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for two point mutations: 462a/g and 663a/g not causing the amino acid variation.

Shipping

10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGCTGAGGG ACACTATGAA ATCTTGGAAT GATAGCCAGT CAGATCTGTG TAGCACTGAC
61       CAAGAAGAGG AAGAAGAGAT GATTTTTGGT GAAAATGAAG ATGATTTGGA TGAGATGATG
121      GATTTAAGTG ATCTGCCTAC CTCACTTTTT GCTTGCAGCG TCCATGAAGC AGTGTTTGAG
181      GCACGAGAGC AGAAGGAAAG ATTTGAAGCA CTCTTCACCA TCTATGATGA CCAGGTTACT
241      TTTCAGCTGT TTAAAAGCTT TAGAAGAGTC AGAATAAATT TCAGCAAACC TGAAGCGGCA
301      GCAAGAGCGC GAATAGAACT CCACGAAACA GACTTCAATG GGCAGAAGCT AAAGCTATAT
361      TTTGCACAGG TGCAGATGTC CGGCGAAGTG CGGGACAAGT CCTATCTCCT GCCGCCCCAG
421      CCTGTCAAGC AGTTCCTCAT CTCCCCTCCA GCCTCTCCCC CGGTGGGGTG GAAGCAGAGC
481      GAAGATGCGA TGCCTGTTAT AAATTATGAT TTACTCTGTG CTGTTTCCAA ATTGGGACCA
541      GGAGAGAAAT ATGAACTTCA CGCGGGAACA GAGTCGACAC CCAGCGTGGT GGTTCATGTC
601      TGTGAAAGTG AAACTGAAGA GGAAGAAGAG ACAAAAAACC CCAAACAGAA AATTGCCCAG
661      ACGAGGCGCC CCGACCCTCC GACCGCAGCG TTGAATGAGC CCCAGACCTT TGATTGCGCG
721      CTGTGA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) RCAN3/DSCR1L2 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)