휴먼(Human) RGM-C transcript variant a 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10410-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_213653.3

cDNA Size:   1281

Synonym:  JH, HJV, RGMC, HFE2A, MGC23953, HFE2

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens hemochromatosis type 2 (juvenile) (HFE2), transcript variant a DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGGGGAGC CAGGCCAGTC CCCTAGTCCC AGGTCCTCCC ATGGCAGTCC CCCAACTCTA
61       AGCACTCTCA CTCTCCTGCT GCTCCTCTGT GGACATGCTC ATTCTCAATG CAAGATCCTC
121      CGCTGCAATG CTGAGTACGT ATCGTCCACT CTGAGCCTTA GAGGTGGGGG TTCATCAGGA
181      GCACTTCGAG GAGGAGGAGG AGGAGGCCGG GGTGGAGGGG TGGGCTCTGG CGGCCTCTGT
241      CGAGCCCTCC GCTCCTATGC GCTCTGCACT CGGCGCACCG CCCGCACCTG CCGCGGGGAC
301      CTCGCCTTCC ATTCGGCGGT ACATGGCATC GAAGACCTGA TGATCCAGCA CAACTGCTCC
361      CGCCAGGGCC CTACAGCCCC TCCCCCGCCC CGGGGCCCCG CCCTTCCAGG CGCGGGCTCC
421      GGCCTCCCTG CCCCGGACCC TTGTGACTAT GAAGGCCGGT TTTCCCGGCT GCATGGTCGT
481      CCCCCGGGGT TCTTGCATTG CGCTTCCTTC GGGGACCCCC ATGTGCGCAG CTTCCACCAT
541      CACTTTCACA CATGCCGTGT CCAAGGAGCT TGGCCTCTAC TGGATAATGA CTTCCTCTTT
601      GTCCAAGCCA CCAGCTCCCC CATGGCGTTG GGGGCCAACG CTACCGCCAC CCGGAAGCTC
661      ACCATCATAT TTAAGAACAT GCAGGAATGC ATTGATCAGA AGGTGTATCA GGCTGAGGTG
721      GATAATCTTC CTGTAGCCTT TGAAGATGGT TCTATCAATG GAGGTGACCG ACCTGGGGGA
781      TCCAGTTTGT CGATTCAAAC TGCTAACCCT GGGAACCATG TGGAGATCCA AGCTGCCTAC
841      ATTGGCACAA CTATAATCAT TCGGCAGACA GCTGGGCAGC TCTCCTTCTC CATCAAGGTA
901      GCAGAGGATG TGGCCATGGC CTTCTCAGCT GAACAGGACC TGCAGCTCTG TGTTGGGGGG
961      TGCCCTCCAA GTCAGCGACT CTCTCGATCA GAGCGCAATC GTCGGGGAGC TATAACCATT
1021     GATACTGCCA GACGGCTGTG CAAGGAAGGG CTTCCAGTGG AAGATGCTTA CTTCCATTCC
1081     TGTGTCTTTG ATGTTTTAAT TTCTGGTGAT CCCAACTTTA CCGTGGCAGC TCAGGCAGCA
1141     CTGGAGGATG CCCGAGCCTT CCTGCCAGAC TTAGAGAAGC TGCATCTCTT CCCCTCAGAT
1201     GCTGGGGTTC CTCTTTCCTC AGCAACCCTC TTAGCTCCAC TCCTTTCTGG GCTCTTTGTT
1261     CTGTGGCTTT GCATTCAGTA A

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) RGM-C transcript variant a 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)