Cynomolgus SFTPD / COLEC7 / Collectin-7 단백질(Protein) (His Tag)

Cynomolgus Surfactant / Pulmonary-Associated Protein D

SFTPD / COLEC7 Protein Product Information

• Synonym: SFTPD
• Protein Construction: A DNA sequence encoding the Cynomolgus ( Macaca fascicularis ) SFTPD ( NP_001035283.1 ) ( Met 1 - Phe 375 ) precursor was expressed, with a C-terminal polyhistidine tag
• Source: Cynomolgus
• Expression Host: Human Cells

SFTPD / COLEC7 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 22
• Molecular Mass: The recombinant cynomolgus SFTPD consists of 365 amino acids and has a calculated molecular mass of 36.8 kDa. The recombinant protein migrates as an approximately 45 kDa in SDS-PAGE under reducing conditions due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE: SFTPD protein

SFTPD / COLEC7 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SFTPD / COLEC7 Protein Description

Cynomolgus surfactant, pulmonary-associated protein D, also known as SFTPD and SP-D, is a member of the collectin family of C-type lectins that is synthesized in many tissues including respiratory epithelial cells in the lung, and contains one C-type lectin domain and one collagen-like domain. SP-D is assembled predominantly as dodecamers consisting of four set of homotrimers stabilized by interchain disulfide bonds involving two conserved amino-terminal cysteine residues. SP-D is produced primarily by alveolar type II cells and nonciliated bronchiolar cells in the lung and is constitutively secreted into the alveoli where it influences surfactant homeostasis, effector cell functions, and host defense. It is upregulated in a variety of inflammatory and infectious conditions including Pneumocystis pneumonia, asthma, and bleomycin injury. Mice or rats exposed to hyperoxic challenge also have increases in SP-D, suggesting that it may have a role in protection from this insult.

References

1. Liu, Y. et al., 1994, J. Immunol. 152:1821-9.
2. Kotenko, SV. et al.,1997, EMBO J. 16 (19): 5894-903.
3. Gibbs, V.C. et al., 1997, Pennica. Gene 186: 97-101.
4. Kotenko, SV. et al., 2001, J. Biol. Chem. 276: 2725-32.
5. Crepaldi, L. et al., 2001, J. Immunol. 167: 2312-22.
6. Dinant, S J. et al., 2007, Surg Res. 141 (2): 176-82.