마우스(Mouse) SIGIRR 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

SIGIRR cDNA Clone Datasheet

SIGIRR cDNA Clone Product Information

• Gene Bank Ref.ID: NM_023059.3
• cDNA Size: 1230
• Gene Synonym: TIR8; AI256711; MGC102426
• Source: Mouse

SIGIRR cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus single immunoglobulin and toll-interleukin 1 receptor (TIR) domain DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

SIGIRR cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SIGIRR cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

SIGIRR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAGGTG TCTGTGACAT GGCCCCTAAT TTCCTTTCCC CATCTGAAGA CCAGGCCTTG
61       GGTCTTGCCC TTGGCAGAGA AGTTGCTTTG AATTGCACAG CTTGGGTGTT CTCTAGGCCC
121      CAGTGTCCCC AGCCATCAGT GCAGTGGCTG AAAGATGGTC TGGCATTGGG CAATGGAAGC
181      CACTTCAGCC TCCATGAGGA CTTCTGGGTC AGCGCCAACT TCTCAGAGAT TGTGTCCAGT
241      GTCCTGGTGC TCAACTTGAC CAATGCAGAG GACTATGGAA CCTTCACCTG TTCTGTCTGG
301      AATGTCAGCT CCCATTCCTT CACTCTTTGG CGAGCTGGCC CTGCTGGCCA TGTGGCTGCA
361      GTACTGGCTT CCCTCCTGGT CCTGGTGGTT CTGCTGCTGG TGGCCCTGCT CTATGTTAAG
421      TGTCGGCTGA ACATGCTGCT TTGGTACCAA GACACTTACG GGGAGGTGGA GATGAACGAT
481      GGGAAGTTAT ACGATGCCTA CGTGTCCTAT AGCGACTGCC CAGAGGACCG CAAATTTGTA
541      AATTTTATTC TGAAGCCTCA GTTGGAGCGG CGTCGGGGAT ACAAACTCTT CCTAGAGGAC
601      CGCGACCTCT TGCCTCGCGC GGAGCCCTCT GCCGACCTTT TGGTGAACCT GAGTCGCTGT
661      CGGCGTCTCA TCGTGGTTCT TTCAGATGCC TTCCTAAGCC GGCCCTGGTG TAGCCAGAGC
721      TTCCGGGAGG GACTGTGCCG CCTACTGGAG CTCACCCGCA GACCTATCTT CATCACCTTT
781      GAGGGCCAGA GGCGTGAGCC CATACACCCT GCTCTCCGGC TCCTGCGCCA GCACCGCCAC
841      CTCGTGACCC TGGTGCTTTG GAAGCCTGGC TCCGTGACTC CTTCCTCTGA TTTTTGGAAA
901      GAGCTACAGC TAGCACTGCC ACGGAAGGTG CAGTACAGGC CGGTGGAGGG AGACCCTCAA
961      ACCCGACTTC AGGATGACAA AGATCCCATG CTAATCGTGA GAGGACGTGC TGCCCAGGGC
1021     CGGGGCATGG AGTCAGAGCT GGATCCAGAC CCTGAGGGAG ACCTGGGTGT CCGTGGACCT
1081     GTCTTTGGGG AGCCACCAAC TCCACTGCAG GAAACCAGGA TCTGCATAGG AGAGAGCCAC
1141     GGCAGTGAAA TGGATGTCTC TGACCTCGGC TCTCGAAACT ACAGTGCACG GACAGACTTC
1201     TACTGCCTCG TGTCTGAGGA TGATGTGTAG

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).