휴먼(Human) SNAP25 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

SNAP25 cDNA Clone Datasheet

SNAP25 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_130811.1
• cDNA Size: 621
• Gene Synonym: SNAP-25, RIC-4, RIC4, SEC9, bA416N4.2, dJ1068F16.2
• Source: Human

SNAP25 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens synaptosomal-associated protein, 25kDa DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

SNAP25 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SNAP25 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

SNAP25 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGAAG ACGCAGACAT GCGCAATGAG CTGGAGGAGA TGCAGCGAAG GGCTGACCAG
61       TTGGCTGATG AGTCGCTGGA AAGCACCCGT CGTATGCTGC AACTGGTTGA AGAGAGTAAA
121      GATGCTGGTA TCAGGACTTT GGTTATGTTG GATGAACAAG GAGAACAACT GGAACGCATT
181      GAGGAAGGGA TGGACCAAAT CAATAAGGAC ATGAAAGAAG CAGAAAAGAA TTTGACGGAC
241      CTAGGAAAAT TCTGCGGGCT TTGTGTGTGT CCCTGTAACA AGCTTAAATC AAGTGATGCT
301      TACAAAAAAG CCTGGGGCAA TAATCAGGAC GGAGTGGTGG CCAGCCAGCC TGCTCGTGTA
361      GTGGACGAAC GGGAGCAGAT GGCCATCAGT GGCGGCTTCA TCCGCAGGGT AACAAATGAT
421      GCCCGAGAAA ATGAAATGGA TGAAAACCTA GAGCAGGTGA GCGGCATCAT CGGGAACCTC
481      CGTCACATGG CCCTGGATAT GGGCAATGAG ATCGATACAC AGAATCGCCA GATCGACAGG
541      ATCATGGAGA AGGCTGATTC CAACAAAACC AGAATTGATG AGGCCAACCA ACGTGCAACA
601      AAGATGCTGG GAAGTGGTTA A

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).