휴먼(Human) STK17B 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

STK17B cDNA Clone Datasheet

STK17B cDNA Clone Product Information

• Gene Bank Ref.ID: NM_004226.3
• cDNA Size: 1119
• Gene Synonym: DRAK2; STK17B
• Source: Human

STK17B cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens serine/threonine kinase 17b DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for four point mutations: 126 A/T, 222 A/G, 741 T/C and 1116T/C not causing the amino acid variation.

STK17B cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

STK17B cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

STK17B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCGAGGA GGAGATTTGA TTGCCGAAGT ATTTCAGGCC TACTAACTAC AACTCCTCAA
61       ATTCCAATAA AAATGGAAAA CTTTAATAAT TTCTATATAC TTACATCTAA AGAGCTAGGG
121      AGAGGTAAAT TTGCTGTGGT TAGACAATGT ATATCAAAAT CTACTGGCCA AGAATATGCT
181      GCAAAATTTC TAAAAAAGAG AAGAAGAGGA CAGGATTGTC GGGCAGAAAT TTTACACGAG
241      ATTGCTGTGC TTGAATTGGC AAAGTCTTGT CCCCGTGTTA TTAATCTTCA TGAGGTCTAT
301      GAAAATACAA GTGAAATCAT TTTGATATTG GAATATGCTG CAGGTGGAGA AATTTTCAGC
361      CTGTGTTTAC CTGAGTTGGC TGAAATGGTT TCTGAAAATG ATGTTATCAG ACTCATTAAA
421      CAAATACTTG AAGGAGTTTA TTATCTACAT CAGAATAACA TTGTACACCT TGATTTAAAG
481      CCACAGAATA TATTACTGAG CAGCATATAC CCTCTCGGGG ACATTAAAAT AGTAGATTTT
541      GGAATGTCTC GAAAAATAGG GCATGCGTGT GAACTTCGGG AAATCATGGG AACACCAGAA
601      TATTTAGCTC CAGAAATCCT GAACTATGAT CCCATTACCA CAGCAACAGA TATGTGGAAT
661      ATTGGTATAA TAGCATATAT GTTGTTAACT CACACATCAC CATTTGTGGG AGAAGATAAT
721      CAAGAAACAT ACCTCAATAT CTCTCAAGTT AATGTAGATT ATTCGGAAGA AACTTTTTCA
781      TCAGTTTCAC AGCTGGCCAC AGACTTTATT CAGAGCCTTT TAGTAAAAAA TCCAGAGAAA
841      AGACCAACAG CAGAGATATG CCTTTCTCAT TCTTGGCTAC AGCAGTGGGA CTTTGAAAAC
901      TTGTTTCACC CTGAAGAAAC TTCCAGTTCC TCTCAAACTC AGGATCATTC TGTAAGGTCC
961      TCTGAAGACA AGACTTCTAA ATCCTCCTGT AATGGAACCT GTGGTGATAG AGAAGACAAA
1021     GAGAATATCC CAGAGGATAG CAGCATGGTT TCCAAAAGAT TTCGTTTCGA TGACTCATTA
1081     CCCAATCCCC ATGAACTTGT TTCAGATTTG CTCTGCTAG

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).